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BS EN ISO 10272-1:2017+A1:2023

BS EN ISO 10272-1:2017+A1:2023

$198.66

Microbiology of the food chain. Horizontal method for detection and enumeration of Campylobacter spp – Detection method. Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp, the use of growth supplement in Preston broth and changes in the performance testing of culture media

Published By Publication Date Number of Pages
BSI 2023 56
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Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media

PDF Catalog

PDF Pages PDF Title
2 undefined
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.2.1 Detection procedure A
4.2.2 Detection procedure B
4.2.3 Detection procedure C
11 4.3 Isolation on selective solid medium
4.3.1 Detection procedure A
4.3.2 Detection procedure B
4.3.3 Detection procedure C
4.3.4 Detection procedure A, B and C
4.4 Confirmation
5 Culture media and reagents
6 Equipment and consumables
12 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
13 9.2 Test portion and initial suspension
9.2.1 General
9.2.2 Detection procedure A
9.2.3 Detection procedure B
9.2.4 Detection procedure C
14 9.3 Enrichment
9.3.1 Detection procedure A
9.3.2 Detection procedure B
9.4 Isolation
9.4.1 Detection procedure A
9.4.2 Detection procedure B
9.4.3 Detection procedures A, B and C
9.5 Confirmation of Campylobacter
9.5.1 General
15 9.5.2 Selection of colonies for confirmation
9.5.3 Examination of morphology and motility
9.5.4 Study of aerobic growth at 25 °C
9.5.5 Detection of oxidase activity
9.5.6 Interpretation
16 9.6 Identification of Campylobacter species (optional)
9.6.1 General
9.6.2 Detection of catalase activity
9.6.3 Detection of hippurate hydrolysis
9.6.4 Detection of indoxyl acetate hydrolysis
17 9.6.5 Interpretation
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
18 12 Test report
19 Annex A (normative) Diagram of procedures
20 Annex B (normative) Culture media and reagents
29 Annex C (informative) Method validation studies and performance characteristics
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52 Bibliography

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BS EN ISO 10272-1:2017+A1:2023
$198.66