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BS EN ISO 16958:2020

BS EN ISO 16958:2020

$198.66

Milk, milk products, infant formula and adult nutritionals. Determination of fatty acids composition. Capillary gas chromatographic method

Published By Publication Date Number of Pages
BSI 2020 56
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This International Standard specifies a method for the quantification of individual and/or all fatty acids in the profile of milk, milk products, infant formula and adult nutritional formula, containing milk fat and/or vegetable oils, supplemented or not supplemented with oils rich in long chain polyunsaturated fatty acids (LC-PUFA). This also includes groups of fatty acids often labelled [i.e. trans fatty acids (TFA), saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), omega-3, omega-6 and omega-9 fatty acids] and/or individual fatty acids [i.e. linoleic acid (LA), α-linolenic acid (ALA), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)].

The determination is performed by direct transesterification in food matrices, without prior fat extraction, and consequently it is applicable to liquid samples or reconstituted powder samples with water having total fat ≥ 1,5 % m/m.

The fat extracted from products containing less than 1,5 % m/m fat can be analysed with the same method after a preliminary fat extraction using methods referenced in Clause 2. Dairy products, like soft or hard cheeses with acidity level ≤ 1 mmol/100 g of fat, can be analysed after a preliminary fat extraction using methods referenced in Clause 2. For products supplemented or enriched with PUFA with fish oil or algae origins, the evaporation of solvents should be performed at the lowest possible temperature (e.g. max. 40 °C) to recover these sensitive fatty acids.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 Forewords
10 1 Scope
2 Normative references
3 Terms and definitions
11 4 Principle
5 Reagents
15 6 Apparatus
17 7 Sampling
18 8 Preparation of test sample
8.1 Liquid and powder milk and infant formula with a fat content ≥ 1,5 % m/m
8.2 Liquid and powder milk and infant formula with a fat content < 1,5 % m/m
8.3 Cheese
9 Procedure
9.1 Test portion
19 9.2 Quantitative determination
9.2.1 Determination of response factors
9.2.2 Determination of the test portion
9.2.3 Fatty acid identification
21 10 Calculation and expression of results
10.1 Calculation
10.1.1 Calculation of response factor
10.1.2 Fatty acids on the product
22 10.1.3 Fatty acids on the total fat
10.1.4 Sum of class or group of fatty acids in 100 g product
10.1.5 Sum of class or group of fatty acids in 100 g fat
10.1.6 Performance of the transesterification
23 10.2 Expression of results
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
24 11.3 Reproducibility
11.4 Limit of detection
11.5 Limit of quantitation
12 Test report
25 Annex A (normative) Groups or classes of fatty acids and individual fatty acids
29 Annex B (informative) Examples of the gas–liquid chromatographic analysis
39 Annex C (informative) Results of an interlaboratory trial
54 Bibliography

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BS EN ISO 16958:2020
$198.66