{"id":425528,"date":"2024-10-20T06:57:21","date_gmt":"2024-10-20T06:57:21","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-21872-12017a12023\/"},"modified":"2024-10-26T13:07:07","modified_gmt":"2024-10-26T13:07:07","slug":"bs-en-iso-21872-12017a12023","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-21872-12017a12023\/","title":{"rendered":"BS EN ISO 21872-1:2017+A1:2023"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
4<\/td>\nEuropean foreword
Endorsement notice <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references <\/td>\n<\/tr>\n
10<\/td>\n3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a liquid selective medium <\/td>\n<\/tr>\n
11<\/td>\n4.3 Secondary enrichment in a liquid selective medium
4.4 Isolation and identification
4.5 Confirmation
5 Culture media and reagents <\/td>\n<\/tr>\n
12<\/td>\n5.1 Enrichment medium: alkaline saline peptone water (ASPW)
5.2 Solid selective isolation media
5.2.1 First medium: thiosulphate, citrate, bile and sucrose agar medium (TCBS)
5.2.2 Second medium
5.3 Saline nutrient agar (SNA)
5.4 Reagent for detection of oxidase
5.5 Biochemical tests
5.5.1 L-lysine decarboxylase saline medium (LDC)
5.5.2 Arginine dihydrolase saline medium (ADH) <\/td>\n<\/tr>\n
13<\/td>\n5.5.3 Reagent for detection of \u03b2-galactosidase
5.5.4 Saline medium for detection of indole
5.5.5 Saline peptone waters
5.5.6 Sodium chloride solution
5.6 PCR
5.6.1 Tris acetate EDTA buffer (TAE) (or a buffer allowing similar performance for the purpose)
5.6.2 Mastermix
5.6.3 Primers and probes
5.6.4 Positive control material <\/td>\n<\/tr>\n
14<\/td>\n5.6.5 Negative extraction control
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample <\/td>\n<\/tr>\n
15<\/td>\n9 Procedure (See Figure A.1)
9.1 Test portion and initial suspension
9.2 Primary selective enrichment
9.3 Secondary selective enrichment <\/td>\n<\/tr>\n
16<\/td>\n9.4 Isolation and identification
9.5 Confirmation
9.5.1 General <\/td>\n<\/tr>\n
17<\/td>\n9.5.2 Selection of colonies for confirmation and preparation of pure cultures
9.5.3 Tests for presumptive identification <\/td>\n<\/tr>\n
18<\/td>\n9.5.4 Biochemical confirmation <\/td>\n<\/tr>\n
20<\/td>\n9.5.5 PCR confirmation
9.5.6 DNA extraction
9.5.7 Conventional PCR <\/td>\n<\/tr>\n
21<\/td>\n9.5.8 Real-time PCR
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study <\/td>\n<\/tr>\n
22<\/td>\n11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report <\/td>\n<\/tr>\n
23<\/td>\nAnnex A (normative) Diagram of procedure <\/td>\n<\/tr>\n
25<\/td>\nAnnex B (normative) Composition and preparation of the culture media and reagents <\/td>\n<\/tr>\n
31<\/td>\nBlank Page <\/td>\n<\/tr>\n
32<\/td>\nBlank Page <\/td>\n<\/tr>\n
33<\/td>\nBlank Page <\/td>\n<\/tr>\n
34<\/td>\nAnnex C (informative) Conventional PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin (tdh) and thermostable direct related haemolysin (trh) genes, Vibrio cholerae and Vibrio vulnificus <\/td>\n<\/tr>\n
38<\/td>\nAnnex D (informative) Real-time PCR for the detection of Vibrio parahaemolyticus, thermostable direct haemolysin gene (tdh) and Vibrio vulnificus <\/td>\n<\/tr>\n
40<\/td>\nAnnex E (informative) Results of an interlaboratory study <\/td>\n<\/tr>\n
43<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. – Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2023<\/td>\n46<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":425536,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-425528","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/425528","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/425536"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=425528"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=425528"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=425528"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}