ASTM-E1397:2008 Edition

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E1397-91(2008) Standard Practice for In Vitro Rat Hepatocyte DNA Repair Assay

Published By	Publication Date	Number of Pages
ASTM	2008	8

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Category: ASTM

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Description

ASTM E1397-91-Reapproved2008

Withdrawn Standard: Standard Practice for In Vitro Rat Hepatocyte DNA Repair Assay (Withdrawn 2014)

ASTM E1397

Scope

1.1 This practice covers a typical procedure and guidelines for conducting the rat in vitro hepatocyte DNA repair assay. The procedures presented here are based on similar protocols that have been shown to be reliable (1-6) .

1.2 Mention of trade names or commercial products are meant only as examples and not as endorsements. Other suppliers or manufacturers of equivalent products are acceptable.

1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

DNA (deoxyribonucleic acid); Genetic examination; Hepatocyte DNA repair assay; Rats; Thymidine

ICS Code

ICS Number Code 07.080 (Biology. Botany. Zoology)

DOI: 10.1520/E1397-91R08

PDF Catalog

PDF Pages	PDF Title
1	Scope Significance and Use Procedure
5	Report
6	A1. SOLUTIONS A1.1 WEI (Williams medium E—incomplete) A1.2 WEC (Williams medium E—complete) A1.3 0.5 mM EGTA Perfusion Solution A1.4 Collagenase Perfusion Solution (100 units/mL) A1.5 3H-Thymidine Solution (10 µCi/mL) A1.6 Methyl-Green Pyronin Y Solution
7	REFERENCES