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ASTM-E1470:1998 Edition

ASTM-E1470:1998 Edition

$35.75

E1470-92(1998) Standard Test Method for Characterization of Proteins by Electrophoretic Mobility

Published By Publication Date Number of Pages
ASTM 1998 2
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ASTM E1470-92-Reapproved1998

Historical Standard: Standard Test Method for Characterization of Proteins by Electrophoretic Mobility

ASTM E1470

Scope

1.1 This test method describes a procedure for determining the electrophoretic mobility of proteins of molecular weight greater than 10000 Daltons.

1.2 This test method uses automatic Electrophoretic Light Scattering (ELS) principles to determine the electrophoretic mobility.

1.3 The instrument simultaneously measures the Doppler shifts of scattered light at four different angles to determine the electrophoretic mobility distribution of protein particles. The mobility is expressed as [mu]m-cm/V-s (micron-centimeter/volt-second).

1.4 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

Collimated laser light; Doppler shifts; Electrophoretic light scattering (ELS); Lasers and laser applications; Light-scattering; Proteins; Zeta potential distribution; electrophoretic mobility of proteins (of molecular weight

ICS Code

ICS Number Code 07.080 (Biology. Botany. Zoology)

DOI: 10.1520/E1470-92R98

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