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ASTM-E2196:2012 Edition

ASTM-E2196:2012 Edition

$40.63

E2196-12 Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown with Medium Shear and Continuous Flow Using Rotating Disk Reactor

Published By Publication Date Number of Pages
ASTM 2012 6
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1.1 This test method is used for growing a reproducible (1) Pseudomonas aeruginosa biofilm in a continuously stirred tank reactor (CSTR) under medium shear conditions. In addition, the test method describes how to sample and analyze biofilm for viable cells.

1.2 Although this test method was created to mimic conditions within a toilet bowl, it can be adapted for the growth and characterization of varying species of biofilm (rotating disk reactor repeatability and relevance ( 2) ).

1.3 This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log 10 colony forming units per surface area (rotating disk reactor efficacy test method ( 3) ).

1.4 Basic microbiology training is required to perform this test method.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

PDF Catalog

PDF Pages PDF Title
1 Scope
Referenced Documents
Terminology
Summary of Test Method
Significance and Use
2 Apparatus
3 Reagents and Materials
FIG. 1
FIG. 2
4 Culture Preparation
Reactor Preparation
Procedure
5 Precision and Bias
Keywords
REFERENCES

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