ASTM-E3029 2015

$40.63

E3029-15 Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers

Published By	Publication Date	Number of Pages
ASTM	2015	5

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Categories: 17.180.01 - Optics and optical measurements in general, ASTM

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Description

ASTM E3029-15

Historical Standard: Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers

ASTM E3029

Scope

1.1 This practice (1)2 describes three methods for determining the relative spectral correction factors for grating-based fluorescence spectrometers in the ultraviolet-visible spectral range. These methods are intended for instruments with a 0°/90° transmitting sample geometry. Each method uses different types of transfer standards, including 1) a calibrated light source (CS), 2) a calibrated detector (CD) and a calibrated diffuse reflector (CR), and 3) certified reference materials (CRMs). The wavelength region covered by the different methods ranges from 250 to 830 nm with some methods having a broader range than others. Extending these methods to the near infrared (NIR) beyond 830 nm will be discussed briefly, where appropriate. These methods were designed for scanning fluorescence spectrometers with a single channel detector, but can also be used with a multichannel detector, such as a diode array or a CCD.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Keywords

calibration; fluorescence; fluorometer; luminescence; qualification; reference materials; spectral correction; spectrometer; spectroscopy; validation;

ICS Code

ICS Number Code 17.180.01 (Optics and optical measurement in general)

DOI: 10.1520/E3029-15

Additional information

Standard Title	E3029-15 Standard Practice for Determining Relative Spectral Correction Factors for Emission Signal of Fluorescence Spectrometers
Published Code	ASTM
Publication Date	2015
Pages Count	5
ICS Codes	17.180.01 - Optics and optical measurements in general