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ASTM-E3143:2023 Edition

ASTM-E3143:2023 Edition

$34.13

E3143-18b(2023) Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

Published By Publication Date Number of Pages
ASTM 2023
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ASTM E3143-18b-Reapproved2023

Active Standard: Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

ASTM E3143

Scope

1.1 This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.

1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Keywords

cryo-TEM; liposomes; vitrificiation;

ICS Code

ICS Number Code 17.180.30 (Optical measuring instruments)

DOI: 10.1520/E3143-18BR23

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