BS EN 12353:2013
$167.15
Chemical disinfectants and antiseptics. Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
| Published By | Publication Date | Number of Pages |
| BSI | 2013 | 38 |
This European Standard specifies methods for keeping test organisms used and defined in European Standards for the determination of bactericidal (incl. Legionella pneumophila), mycobactericidal, sporicidal, fungicidal and virucidal (incl. bacteriophages) activity of chemical disinfectants and antiseptics drawn up by CEN/TC 216. These methods for keeping test organisms can only be carried out in connection with at least one of those standards where a reference to this European Standard is established. NOTE 1 Annex A (informative) contains a non-exhaustive list of test organisms for which this standard can be applied. NOTE 2 European Standards (EN and prEN) where this European Standard is referenced are listed in the Bibliography. NOTE 3 A specific part on the preservation of bacterial spores may be added once the results of the ongoing ring trials are available.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 7 | 1 Scope 2 Normative references 3 Terms and definitions 4 Requirements 5 Methods 5.1 Principle |
| 8 | 5.2 Materials and reagents 5.2.1 Test organisms 5.2.2 Culture media and reagents 5.2.2.1 General 5.2.2.2 Water 5.2.2.3 Tryptone Soya Broth (TSB) for bacteria, except Legionella |
| 9 | 5.2.2.4 Malt Extract Broth (MEB) for fungi 5.2.2.5 Cryoprotectant solution for bacteria, spore-forming bacteria, fungi 5.2.2.6 Middlebrook 7 H 9 broth with 10 % ADC enrichment and glycerol as reconstituent and cryoprotectant solution for mycobacteria (MADC) 5.2.2.7 Polysorbate 80 solution |
| 10 | 5.2.2.8 DMSO as cryoprotectant for cell culture freezing 5.2.2.9 Glutamine solution, 3 % 5.2.2.10 TV (Trypsin-Versene) 5.2.2.11 Antibiotic suspension 5.2.2.12 Phosphate-buffered saline solution (PBS) 5.2.2.13 Foetal calf serum (FCS) 5.2.2.14 Earle’s BSS |
| 11 | 5.2.2.15 Phenol red, 1 % solution 5.2.2.16 Sodium bicarbonate (8,8 % w/v solution) 5.2.2.17 Eagle’s minimum essential medium (MEM) for cell cultures |
| 13 | 5.2.2.18 M17-broth 5.2.2.19 M17-agar (bottom agar) 5.2.2.20 Overlay agar (top agar, soft agar) |
| 14 | 5.2.2.21 CaCl2-stock solutions (1 mol/l and 0,05 mol/l) 5.2.2.22 Skim milk 5.2.2.23 Buffered Charcoal Yeast Extract (BCYE) Broth, for Legionella |
| 15 | 5.2.2.24 Page’s saline 5.2.3 Cell cultures 5.2.3.1 Storage of cell cultures |
| 16 | 5.2.3.2 Preparation of new cell cultures 5.2.4 Host strains for dairy bacteriophages (Lactococcus lactis) 5.2.4.1 Stock culture of host bacteria |
| 17 | 5.2.4.2 Working culture of host bacteria 5.3 Apparatus and glassware 5.3.1 General 5.3.2 Usual microbiological laboratory equipment3F |
| 18 | 5.3.2.17 Biological safety cabinet, class II or higher, for growing cell cultures and viruses. 5.4 Procedure for preservation of test organisms – General 5.4.1 Handling of freeze dried / frozen test organisms from culture collections 5.4.2 Choice of incubation procedure, agar medium, cell culture/cell line |
| 19 | 5.5 Procedure for preservation of bacteria (incl. Legionella, spore-forming bacteria, excl. mycobacteria and bacterial spores) and yeasts5F 5.5.1 Reconstitution of the freeze dried test organisms 5.5.2 Preparation for storage 5.5.3 Preparation of stock culture / working cultures |
| 20 | 5.6 Procedure for preservation of mycobacteria6F 5.6.1 Reconstitution of the freeze dried test organisms 5.6.2 Preparation for storage 5.6.3 Preparation of working cultures |
| 21 | 5.7 Procedure for preservation of moulds (e.g. Aspergillus brasiliensis)7F 5.7.1 Reconstitution of the freeze dried test organism 5.7.2 Preparation for storage 5.7.3 Preparation of stock culture / working cultures |
| 22 | 5.8 Procedure for preservation of viruses (except dairy bacteriophages)8F 5.8.1 Reconstitution of frozen virus 5.8.2 Preparation for storage of stock virus suspension 5.8.3 Preparation of test virus suspension 5.9 Procedure for preservation of bacteriophages 5.9.1 Reconstitution of frozen bacteriophages |
| 23 | 5.9.2 Preparation for storage 5.10 Verification of the purity and identity of test organisms 5.10.1 General 5.10.2 Purity 5.10.3 Identity 5.11 Documentation |
| 24 | 5.11.1 Freeze dried test organism / frozen viruses 5.11.2 Cryovials of frozen test organism 5.11.3 Stock culture 5.11.4 Verification of purity and identity |
| 25 | Annex A (informative) Test organisms – Culture collection references and relation to CEN/TC 216 standards and prENs A.1 Bacteria (except mycobacteria and spore-forming bacteria) A.1.1 Enterobacter cloacae A.1.2 Enterococcus hirae A.1.3 Enterococcus faecium A.1.4 Escherichia coli (1) A.1.5 Escherichia coli (2) K12 A.1.6 Lactobacillus brevis A.1.7 Legionella pneumophila, serogroup 1, Philadelphia Culture collections: NCTC 11192, ATCC 33152, CIP 103854 T |
| 26 | A.1.8 Proteus hauseri A.1.9 Pseudomonas aeruginosa A.1.10 Salmonella enterica subsp. enterica, Serotype typhimurium A.1.11 Staphylococcus aureus subsp. aureus A.2 Mycobacteria A.2.1 Mycobacterium avium subsp. avium A.2.2 Mycobacterium terrae A.3 Spore-forming bacteria A.3.1 Bacillus cereus |
| 27 | A.3.2 Bacillus subtilis subsp. spizizenii A.3.3 Clostridium sporogenes A.4 Fungi (moulds and yeasts) A.4.1 Aspergillus brasiliensis (former “A. niger”) (mould) A.4.2 Candida albicans (yeast) A.4.3 Saccharomyces cerevisiae (1) (yeast) A.4.4 Saccharomyces cerevisiae (2) var. diastaticus (yeast) A.5 Viruses A.5.1 Adenovirus type 5, strain Adenoid 75 |
| 28 | A.5.2 Bovine Enterovirus Type 1 (ECBO) A.5.3 Bovine Parvovirus, strain Haden A.5.4 Poliovirus type 1, Sabin strain LSc-2ab A.6 Bacteriophages A.6.1 Lactococcus lactis subsp. lactis bacteriophage P008 A.6.2 Lactococcus lactis subsp. lactis bacteriophage P001 |
| 29 | Annex B (informative) Graphical representations |
