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BS EN 13697:2015

$167.15

Chemical disinfectants and antiseptics. Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas. Test method and requirements without mechanical action (phase 2, step 2)

Published By Publication Date Number of Pages
BSI 2015 38
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This European Standard specifies a test method (phase 2/step 2) and the minimum requirements for bactericidal and/or fungicidal or yeasticidal activity of chemical disinfectants that form a homogeneous physically stable preparation in hard water or – in the case of ready-to-use products – with water in food, industrial, domestic and institutional areas, excluding areas and situations where disinfection is medically indicated and excluding products used on living tissues. The scope of this European Standard applies at least to the following: a) Processing, distribution and retailing of: 1) Food of animal origin: i) milk and milk products; ii) meat and meat products; iii) fish, seafood and products; iv) eggs and egg products; v) animal feeds; vi) etc. 2) Food of vegetable origin: i) beverages; ii) fruits, vegetables and derivatives (including sugar distillery); iii) flour, milling and backing; iv) animal feeds; v) etc. b) Institutional and domestic areas: 1) catering establishments; 2) public areas; 3) public transports; 4) schools; 5) nurseries; 6) shops; 7) sports rooms; 8) waste container (bins); 9) hotels; 10) dwellings; 11) clinically non sensitive areas of hospitals; 12) offices; 13) etc. c) Other industrial areas: 1) packaging material; 2) biotechnology (yeast, proteins, enzymes…); 3) pharmaceutical; 4) cosmetics and toiletries; 5) textiles; 6) space industry, computer industry; 7) etc. Using this European Standard, it is possible to determine the bactericidal or fungicidal or yeasticidal activity of the undiluted product. As three concentrations are tested, in the active to non active range, dilution of the product is required and, therefore, the product forms a homogeneous stable preparation in hard water. EN 14885 specifies in detail the relationship of the various tests to one another and to use recommendations. NOTE 1 The method described is intended to determine the activity of commercial formulations or active substances on bacteria and/or fungi in the conditions in which they are used. NOTE 2 This method cannot be used to evaluate the activity of products against mycobacteria.

PDF Catalog

PDF Pages PDF Title
4 Contents Page
7 1 Scope
8 2 Normative references
3 Terms and definitions
9 4 Requirements
10 5 Test methods
5.1 Principle
5.2 Materials and reagents
5.2.1 Test organisms
11 5.2.2 Culture media and reagents
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Tryptone Soya Agar (TSA)
5.2.2.4 Malt extract agar (MEA)
12 5.2.2.5 Diluent
5.2.2.6 Neutralizer
5.2.2.7 Hard water for dilution of the products
13 5.2.2.8 Interfering substances
14 5.2.2.9 Sodium lauryl sulphate (cosmetics or cosmetic industries)
5.2.3 Test surface
15 5.3 Apparatus and glassware
16 5.4 Preparation of test organism suspensions and product test solutions
5.4.1 Test organism suspensions
5.4.1.1 Stock cultures of test organisms
5.4.1.2 Working culture of test organisms
17 5.4.1.3 Test suspensions
18 5.4.1.4 Counting of bacterial and fungal test suspensions
19 5.4.1.5 Counting the weighed mean of the test suspensions
5.4.2 Product test solutions
5.5 Procedure
5.5.1 Choice of experimental conditions
20 5.5.2 Test procedure
5.5.2.1 Test “Nd” – determination of microbicidal concentrations
21 5.5.2.2 Water control “Nc”
22 5.5.2.3 Neutralizer control “NC” – verification of the absence of toxicity of the neutralizer
5.5.2.4 Method validation “NT” – dilution-neutralization validation
5.5.3 Counting of the test mixtures
23 5.6 Calculation and expression of results
5.6.1 Elaboration of data: counting of weighed average values
24 5.6.2 Verification of methodology
25 5.6.3 Expression of results
5.6.3.1 Reduction
5.6.4 Conclusion
5.6.4.1 Activity on non-porous surfaces for general purposes
5.6.4.2 Activity on non-porous surfaces for specific purposes
26 5.7 Test report
28 Annex A (informative)Corresponding referenced strains
30 Annex B (informative)Neutralizers
32 Annex C (informative)Expression of results with the dilution-neutralization method
34 Annex D (informative)Bactericidal activity in surfaces in general use conditions (for clean conditions)
35 Annex E (informative)Precision of the test result
BS EN 13697:2015
$167.15