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BS EN 14735:2021

BS EN 14735:2021

$198.66

Characterization of waste. Preparation of waste samples for ecotoxicity tests

Published By Publication Date Number of Pages
BSI 2021 60
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This document describes the necessary steps to be performed before carrying out ecotoxicity tests on wastes. The purpose of this document is to provide guidance on the taking of the sample, transport, storage of wastes and to define preparation, for the determination of ecotoxicological properties of wastes under the conditions specified in this document by biological testing either as raw wastes or water extracts from wastes. Sample preparation for other applications (e.g. assessment of waste effects on aquatic and terrestrial organisms in a disposal scenario) is not considered. Specifying a test battery to characterize ecotoxicological properties of wastes is not in the scope of this document. This document is applicable to solid and liquid wastes.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
9 3 Terms and definitions
11 4 Equipment and reagents
5 Taking of laboratory sample
12 6 Transport
7 Storage
7.1 General
7.2 Waste sample
7.3 Water extracts
13 8 Waste characterization
9 Waste pre-treatment: particle size reduction (granular waste, monolithic waste, paste-like waste and sludge)
14 10 Tests performed on terrestrial organisms
10.1 General considerations
15 10.2 Dilution medium
10.3 Introduction of waste into the dilution medium
10.3.1 General
10.3.2 Monolithic waste, granular waste, paste-like waste and sludge
10.3.3 Liquid sludge
16 10.3.4 Liquid waste miscible with water
10.3.5 Liquid waste non-miscible with water
10.4 Water extracts of waste
10.5 pH
17 10.6 Addition of test organisms
11 Tests performed on aquatic organisms
11.1 General considerations
11.2 Monolithic waste, granular waste, paste-like waste and sludge
11.2.1 Leaching procedure
18 11.2.2 Liquid/solid separation procedure
11.2.3 pH
19 11.3 Liquid sludge
11.3.1 Procedure
11.3.2 pH
11.4 Liquid waste miscible with water
11.4.1 Procedure
11.4.2 pH
11.5 Liquid waste non-miscible with water
11.5.1 Procedure
20 11.5.2 Liquid/liquid separation
11.5.3 pH
11.6 Preparation of test mixtures
12 Test report
21 Annex A (normative)Preparation of test mixtures according to the ecotoxicity tests to be performed
22 Annex B (informative)Ecotoxicity tests considered to establish this document
B.1 General
23 B.2 Terrestrial tests methods
B.2.1 Earthworms – Acute toxicity
24 B.2.2 Earthworms – Effects on reproduction
25 B.2.3 Earthworms – Avoidance
26 B.2.4 Collembola – Effects on reproduction
27 B.2.5 Coleoptera – Acute test
28 B.2.6 Enchytraeid – Reproduction test
29 B.2.7 Nematoda – Effects on growth, fertility and reproduction
30 B.2.8 Juvenile land snails (Helix aspersa)
31 B.2.9 Soil Flora – Inhibition of root growth
33 B.2.10 Effects on emergence and growth
35 B.2.11 Ammonium oxidation – Rapid test
36 B.2.12 Mineralization and nitrification
37 B.2.13 Dehydrogenase activity of Arthrobacter globiformis
39 B.3 Aquatic tests methods
B.3.1 Daphnia magna – Inhibition of mobility
40 B.3.2 Daphnia magna – Inhibition of reproduction
41 B.3.3 Ceriodaphnia dubia reproduction test
42 B.3.4 Brachionus calyciflorus reproduction test
43 B.3.5 Aliivibrio fischeri – Luminescent bacteria test
44 B.3.6 Pseudomonas putida growth inhibition test
45 B.3.7 Freshwater algal growth inhibition test
47 B.3.8 Lemna minor – Growth inhibition test
48 B.3.9 Fish egg test
49 B.3.10 Freshwater fish acute toxicity test
50 B.3.11 Marine copepods – Acute toxicity test
51 B.3.12 Marine algal growth inhibition test
53 B.3.13 Salmonella / Microsome test
55 B.3.14 UMU test

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BS EN 14735:2021
$198.66