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BS EN 15784:2021

BS EN 15784:2021

$102.76

Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Bacillus spp. used as feed additive

Published By Publication Date Number of Pages
BSI 2021 20
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This document specifies general rules for the enumeration of bacilli in feeding stuffs (additives, premixtures and compound feeds including mineral feeds) [4] that contain bacilli as a single microorganism component or in a mixture with other microorganisms. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing about 1011 CFU/kg; c) Compound feeds, meal or pellets containing about 109 CFU/kg.

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
8 5 Diluent and culture medium
5.1 Diluent
5.2 Culture medium tryptone soy agar (TSA)
5.2.1 Composition
9 5.2.2 Preparation
6 Apparatus
10 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Preparation of poured agar plates for spread plate method
11 9.2 Preparation of the initial suspension and decimal dilutions
12 9.3 Inoculation and incubation of plates
9.4 Enumeration of colonies
13 9.5 Confirmation
10 Expression of results
14 11 Precision
11.1 General
11.2 Interlaboratory studies
11.3 Repeatability
11.4 Reproducibility
12 Test report
15 Annex A (informative)Notes on the procedure
16 Annex B (informative)Results of the interlaboratory studies
B.1 General
B.2 Data obtained from VDLUFA collaborative studies

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BS EN 15784:2021
$102.76