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BS EN 15787:2021

BS EN 15787:2021

$142.49

Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Lactobacillus spp. used as feed additive

Published By Publication Date Number of Pages
BSI 2021 24
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This document specifies general rules for the enumeration of lactobacilli in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain lactobacilli as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds, which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) No 767/2009) [3]. There are different categories of feed samples: a) Additives containing about 1010 colony forming units (CFU)/g; b) Premixtures containing about 1011 CFU/kg; c) Compound feeds, meal or pellets which contain about 109 CFU/kg.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
9 5 Diluents and culture media
5.1 Diluents
5.1.1 Diluent for initial suspension
10 5.1.2 Diluents for serial dilutions
5.2 Culture media
5.2.1 General
5.2.2 Composition
11 5.2.3 Preparation
12 6 Apparatus
13 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Samples with a single or multi-component added microflora
9.2 Preparation of poured agar plates for spread plate method
14 9.3 Preparation of MRS agar for pour plate method
9.4 Preparation of the initial suspension and decimal dilutions
15 9.5 Inoculation and incubation of plates
9.5.1 General
9.5.2 Spread plate method
16 9.5.3 Pour plate method
9.6 Enumeration of colonies
9.7 Confirmation
17 10 Expression of results
11 Precision
11.1 General
11.2 Interlaboratory study
11.3 Repeatability
18 11.4 Reproducibility
12 Test report
19 Annex A (informative)Notes on the procedure
A.1 General
A.2 Critical copper concentration
21 Annex B (informative)Results of the interlaboratory study
B.1 General
B.2 Results of the interlaboratory study

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BS EN 15787:2021
$142.49