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BS EN 16187:2015 – TC:2020 Edition

BS EN 16187:2015 – TC:2020 Edition

$186.33

Tracked Changes. Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after pre-column derivatisation

Published By Publication Date Number of Pages
BSI 2020 49
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This European Standard specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112 µg/kg to 458 µg/kg for FB1+FB2, 89 µg/kg to 384 µg/kg for FB1 and 22 µg/kg to 74 µg/kg for FB2. For further information on the validation, see Clause 8 and Annex B.

PDF Catalog

PDF Pages PDF Title
30 Foreword
31 1 Scope
2 Normative references
3 Principle
4 Reagents
33 Table 1 — Preparation of mixed FBs calibration solutions for HPLC
34 5 Apparatus
35 Table 2 — Gradient conditions
6 Procedure
6.1 Extraction
36 6.2 Immunoaffinity column cleanup
6.3 Spiking procedure
6.4 Derivatisation and HPLC determination
6.4.1 Automated pre-column derivatisation programme
37 6.4.2 HPLC injections
6.4.3 Peak identification
6.4.4 Determination
7 Calculation
38 8 Precision
8.1 General
8.2 Repeatability
39 8.3 Reproducibility
40 9 Test report
41 Annex A (informative) Typical chromatograms
Figure A.1 — Typical chromatogram of baby food (pre-cooked flours of diastased maize and tapioca) spiked with 250,0 µg/kg fumonisin B1 (FB1) and 62,5 µg/kg fumonisin B2 (FB2)
Figure A.2 — Typical chromatogram of baby food (biscuits containing wheat, barley, rye, rice and maize) naturally contaminated with 164,8 µg/kg FB1 and 35,6 µg/kg FB2
42 Annex B (informative) Precision data
Table B.1 — Precision and recovery data for fumonisins B1+B2 in baby food
43 Table B.2 — Precision and recovery data for fumonisin B1 in baby food
44 Table B.3 — Precision and recovery data for fumonisin B2 in baby food
45 Annex C (informative) Comparison between the method in this document and EN 14352:2004 and EN 13585:2001 on fumonisins in maize
Table C.1 — Comparison between the method in this document and EN 14352:2004 and EN 13585:2001
46 Bibliography

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BS EN 16187:2015 - TC
$186.33