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BS EN 17547:2021

BS EN 17547:2021

$167.15

Animal feeding stuffs: Methods of sampling and analysis. Determination of vitamin A, E and D content. Method using solid phase extraction (SPE) clean-up and highperformance liquid chromatography (HPLC)

Published By Publication Date Number of Pages
BSI 2021 42
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This European Standard specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (alpha-tocopherol) and vitamin D (D2 ergocalciferol or D3 cholecalciferol) content in animal feed using solid phase extraction (SPE) clean-up and high performance liquid chromatography (HPLC). The limit of quantification is XXXX IU vitamin A/kg (using UV-detection), XX IU vitamin A/kg (using fluorescence detection), XX mg vitamin E/kg (using UV-detection), XX mg vitamin E/kg (using fluorescence detection), XX IU vitamin D/kg (using UV-detection) and XX IU vitamin D/kg (using fluorescence detection).

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principle
10 5 Reagents and materials
11 6 Apparatus
12 7 Sampling
13 8 Sample preparation
9 Procedure
23 10 Expression of results
26 11 Observations
27 12 Precision
28 13 Test report
29 Annex A (informative)Examples of combinations of weighing, aliquot and dilution to reach concentrations within the calibration curve
32 Annex B (informative) Preparation of stock standard solution of vitamin E (α-tocopherol) from α-tocopherol acetate
B.1 General
B.2 Reagents
B.3 Preparation of stock standard
B.4 Standardization of the vitamin E (α-tocopherol) stock standard solution in cyclohexane
33 B.5 Calibration solutions and plotting of calibration graph for vitamins A (retinol) and E (α-tocopherol)
34 Annex C (informative)Results of the interlaboratory study

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BS EN 17547:2021
$167.15