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BS EN ISO 10253:2024

BS EN ISO 10253:2024

$167.15

Water quality. Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum

Published By Publication Date Number of Pages
BSI 2024 34
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This document specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.). The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium. NOTE            With modifications, as described in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, metal compounds, effluents, marine water samples and elutriates of sediments can be tested.

PDF Catalog

PDF Pages PDF Title
2 undefined
8 5.1 Test organisms
10 5.2 Reagents
5.2.1 Water
5.2.2 Hydrochloric acid or sodium hydroxide solution
5.2.3 Synthetic sea water
5.2.4 Nutrient stock solution
12 7.1 Preparation of growth medium
7.2 Preparation of pre-culture and inoculum
7.3 Choice of test concentrations
7.4 Preparation of test substance stock solutions
13 7.5 Preparation of test and control batches
7.6 Incubation
14 7.7 Measurements
9.1 Plotting growth curves
9.2 Calculation of percentage inhibition
15 9.3 Determination of EC(r)x

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BS EN ISO 10253:2024
$167.15