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BS EN ISO 11816-1:2013

BS EN ISO 11816-1:2013

$142.49

Milk and milk products. Determination of alkaline phosphatase activity – Fluorimetric method for milk and milk-based drinks

Published By Publication Date Number of Pages
BSI 2013 24
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This part of ISO 11816|IDF 155 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.

The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk (7.1) so as to obtain a measurement not higher than 7 000 mU/l.

PDF Catalog

PDF Pages PDF Title
4 Foreword
7 Foreword
9 Section sec_1
Section sec_2
Section sec_2.1
Section sec_2.2
Section sec_3
Section sec_4
1 Scope
2 Terms and definitions
3 Principle
4 Reagents
10 Section sec_4.1
Section sec_4.2
Section sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.3
Section sec_4.5
Section sec_5
5 Apparatus
11 Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.4
Section sec_5.5
Section sec_5.6
Section sec_5.7
Section sec_5.8
Section sec_6
Section sec_7
Section sec_7.1
6 Sampling
7 Preparations
7.1 Alkaline phosphatase-free milk
12 Section sec_7.2
Section sec_7.2.1
Section sec_7.2.2
Section sec_7.2.3
Section sec_8
Section sec_8.1
Section sec_8.1.1
Section sec_8.1.2
Section sec_8.1.2.1
Section sec_8.1.2.2
Section sec_8.1.2.3
7.2 Preparation of test sample
8 Procedure
8.1 Verification of instrument performance
13 Section sec_8.1.3
Section sec_8.2
Section sec_8.3
8.2 Reagent controls to test the suitability of ready-to-use working substrate (4.3)
8.3 Calibration
14 Section sec_8.4
Section sec_8.5
Section sec_8.5.1
Section sec_8.5.1.1
Section sec_8.5.1.2
8.4 Determination
8.5 Test sample-related controls
15 Section sec_8.5.2
Section sec_8.5.3
Section sec_9
Section sec_9.1
9 Calculation and expression of results
9.1 Calibration ratio
16 Section sec_9.2
Section sec_9.3
Section sec_10
Section sec_10.1
Section sec_10.2
Section sec_10.3
9.2 Calculation
9.3 Expression of test results
10 Precision
10.1 Collaborative test
10.2 Repeatability
10.3 Reproducibility
17 Section sec_11
11 Test report
18 Annex sec_A
Table tab_A.1
AnnexĀ A
(informative)

Collaborative trials

19 Table tab_A.2
Table tab_A.3
20 Table tab_A.4
Table tab_A.5
21 Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Bibliography

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BS EN ISO 11816-1:2013
$142.49