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BS EN ISO 14852:2021

$142.49

Determination of the ultimate aerobic biodegradability of plastic materials in an aqueous medium. Method by analysis of evolved carbon dioxide

Published By Publication Date Number of Pages
BSI 2021 28
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This document specifies a method, by measuring the amount of carbon dioxide evolved, for the determination of the degree of aerobic biodegradability of plastic materials, including those containing formulation additives. The test material is exposed in a synthetic medium under standardized laboratory conditions to an inoculum from activated sludge under aerobic conditions.

The conditions used in this document do not necessarily correspond to the optimum conditions allowing maximum biodegradation to occur, but this test method is designed to measure the biodegradation of plastic materials and give an indication of their potential biodegradability.

The method enables the assessment of the biodegradation to be improved by calculating a carbon balance (optional, see Annex C).

The method applies to the following materials:

  • natural and/or synthetic polymers, copolymers or mixtures thereof;

  • plastic materials which contain additives such as plasticizers, colorants or other compounds;

  • water-soluble polymers;

  • materials which, under the test conditions, do not inhibit the microorganisms present in the inoculum. Inhibitory effects can be determined using an inhibition control or by another appropriate method (see, for example, ISO 8192 [ 1 ]). If the test material is inhibitory to the inoculum, a lower test concentration, another inoculum or a pre-exposed inoculum can be used.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
11 4 Principle
12 5 Test environment
6 Reagents
14 7 Apparatus
15 8 Procedure
8.1 Test material
8.2 Reference material
8.3 Preparation of the inoculum
8.3.1 General
16 8.3.2 Inoculum from wastewater-treatment plants
8.4 Test
17 9 Calculation and expression of results
9.1 Calculation
9.1.1 Theoretical amount of carbon dioxide evolved by the test material
18 9.1.2 Percentage biodegradation from CO2 evolution
9.2 Expression and interpretation of results
19 10 Validity of results
11 Test report
21 Annex A (informative) Principle of a system for measuring evolved carbon dioxide (example)
22 Annex B (informative) Examples of methods for the determination of evolved carbon dioxide
24 Annex C (informative) Example of the determination of a carbon balance
26 Annex D (informative) Example of a determination of the amount of water insoluble polymer remaining at the end of a biodegradation test and the molecular mass of the polymer
27 Bibliography
BS EN ISO 14852:2021
$142.49