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BS EN ISO 18415:2017 – TC:2020 Edition

BS EN ISO 18415:2017 – TC:2020 Edition

$186.33

Tracked Changes. Cosmetics. Microbiology. Detection of specified and non-specified microorganisms

Published By Publication Date Number of Pages
BSI 2020 61
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PDF Catalog

PDF Pages PDF Title
33 National foreword
38 Foreword
39 Introduction
40 1 Scope
2 Normative references
3 Terms and definitions
42 4 Principle
5 Diluents and culture media
5.1 General
5.2 Diluent for the microbial suspension (tryptone sodium chloride solution)
5.2.1 General
43 5.2.2 Composition
5.2.3 Preparation
5.3 Culture media
5.3.1 General
5.3.2 Enrichment broth
44 5.3.3 Non-selective agar medium
6 Apparatus and glassware
45 7 Strains of microorganism
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendations
9.2 Preparation of the initial suspension in the enrichment broth
9.2.1 General
46 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.2.4 Filterable products
9.3 Incubation of the initial suspension
9.4 Isolation of specified and non-specified microorganisms
9.5 Procedure for identification of the specified microorganism: Pseudomonas aeruginosa
9.5.1 Gram staining
9.5.2 Oxidase test
47 9.5.3 Identification test
9.6 Procedure for identification of the specified microorganism: Escherichia coli
9.6.1 Gram staining
9.6.2 Oxidase test
9.6.3 Identification test
9.7 Procedure for identification of the specified microorganism: Staphylococcus aureus
9.7.1 Gram staining
9.7.2 Catalase test
9.7.3 Identification test
48 9.8 Procedure for the identification of the specified microorganism: Candida albicans
9.8.1 Gram staining
9.8.2 Identification test
9.9 Procedure for the identification of non-specified microorganisms
9.9.1 Gram staining
9.9.2 Oxidase test
9.9.3 Catalase test
9.9.4 Identification test
49 10 Expression of the results
10.1 Detection of specified microorganisms
10.2 Detection of non-specified microorganisms
10.3 Absence of microorganisms
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
11.3 Suitability of detection method by enrichment
11.3.1 Principle
50 11.3.2 Procedure
11.3.3 Interpretation of suitability test results
12 Test report
52 Annex A (informative) General scheme for identification of microorganisms
53 Annex B (informative) Other media
56 Annex C (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
58 Bibliography

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BS EN ISO 18415:2017 - TC
$186.33