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BS EN ISO 18415:2017+A1:2022

$142.49

Cosmetics. Microbiology. Detection of specified and non-specified microorganisms

Published By Publication Date Number of Pages
BSI 2022 32
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PDF Catalog

PDF Pages PDF Title
2 undefined
5 European foreword
Endorsement notice
6 Blank Page
9 Foreword
10 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
13 4 Principle
5 Diluents and culture media
5.1 General
5.2 Diluent for the microbial suspension (tryptone sodium chloride solution)
5.2.1 General
14 5.2.2 Composition
5.2.3 Preparation
5.3 Culture media
5.3.1 General
5.3.2 Enrichment broth
15 5.3.3 Non-selective agar medium
6 Apparatus and glassware
16 Blank Page
17 7 Strains of microorganism
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendations
9.2 Preparation of the initial suspension in the enrichment broth
9.2.1 General
18 9.2.2 Water-miscible products
9.2.3 Water-immiscible products
9.2.4 Filterable products
9.3 Incubation of the initial suspension
9.4 Isolation of specified and non-specified microorganisms
9.5 Procedure for identification of the specified microorganism: Pseudomonas aeruginosa
9.5.1 Gram staining
9.5.2 Oxidase test
19 9.5.3 Identification test
9.6 Procedure for identification of the specified microorganism: Escherichia coli
9.6.1 Gram staining
9.6.2 Oxidase test
9.6.3 Identification test
9.7 Procedure for identification of the specified microorganism: Staphylococcus aureus
9.7.1 Gram staining
9.7.2 Catalase test
9.7.3 Identification test
20 9.8 Procedure for the identification of the specified microorganism: Candida albicans
9.8.1 Gram staining
9.8.2 Identification test
9.9 Procedure for the identification of non-specified microorganisms
9.9.1 Gram staining
9.9.2 Oxidase test
9.9.3 Catalase test
9.9.4 Identification test
21 10 Expression of the results
10.1 Detection of specified microorganisms
10.2 Detection of non-specified microorganisms
10.3 Absence of microorganisms
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
11.3 Suitability of detection method by enrichment
11.3.1 Principle
22 11.3.2 Procedure
11.3.3 Interpretation of suitability test results
12 Test report
24 Annex A (informative) General scheme for identification of microorganisms
25 Annex B (informative) Other media
28 Annex C (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids
30 Bibliography
BS EN ISO 18415:2017+A1:2022
$142.49