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BS EN ISO 19020:2017

$142.49

Microbiology of the food chain. Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs

Published By Publication Date Number of Pages
BSI 2017 30
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This document specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.

This document is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.

Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, this document is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
Endorsement notice
6 Foreword
7 Introduction
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Principle
5 Reagents
6 Apparatus
10 7 Sampling
8 Procedure
8.1 Preparation of test portion
8.2 Storage of the test sample
11 8.3 Extraction
12 8.4 Concentration of the extract (mandatory for milk and dairy products)
8.5 Recovery of the concentrated extract
13 8.6 Storage and steps before detection
8.7 Detection
8.8 Performance criteria
9 Quality control
14 10 Expression of results
11 Confirmation
12 Performance characteristics of the method
16 13 Test report
17 Annex A (informative) Results of interlaboratory studies: 2013
22 Annex B (informative) Results of interlaboratory studies: 2014
28 Annex C (informative) Note on interferences
29 Bibliography
BS EN ISO 19020:2017
$142.49