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BS EN ISO 22579:2021

BS EN ISO 22579:2021

$142.49

Infant formula and adult nutritionals. Determination of fructans. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment

Published By Publication Date Number of Pages
BSI 2021 32
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This document specifies a method for the determination of inulin-type fructans (including oligofructose, fructooligosaccharides) in infant formula and adult nutritionals (both powder and liquid) containing 0,03 g/100 g to 5,0 g/100 g of fructans in the product as prepared ready for consumption.

The method has been validated in a multi laboratory study[ 1] with reconstituted standard reference material (SRM), infant/adult nutritional formula at a level of 0,204 g/100 g, adult nutritionals ready-to-feed (RTF) at levels of 1,28 g/100 g and 2,67 g/100 g, infant formula RTF at a level of 0,300 g/100 g, reconstituted follow-up formula at levels of 0,209 g/100 g to 0,275 g/100 g, reconstituted infant formula at levels from 0,030 8 g/100 g to 0,264 g/100 g. During the single laboratory validation study[ 2], spike-recovery experiments were performed up to 5 g/100 g in reconstituted infant formula powders (milk-based, partially hydrolysed milk-based and soy-based), adult nutritional RTF and reconstituted adult nutritional powders.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
5 Chemicals and reagents
5.1 List of chemicals and reagents
11 5.2 Preparation of reagents
12 5.2.7 Carrez I solution
5.2.8 Carrez II solution
13 5.3 Preparation of mobile phases using column A (6.13.1) or equivalent
5.4 Preparation of mobile phases using columns B (6.13.2) or equivalent
14 5.5 Preparation of standard solutions
6 Apparatus
16 7 Procedure
7.1 Sample preparation
7.1.1 Powdered or concentrated products on a ready-to feed (RTF) basis and powder products inhomogeneous at the subgram level
7.1.2 Reconstituted products as prepared in 7.1.1 or products sold as RTF
7.1.3 Homogeneous powdered products without prior reconstitution
7.1.4 Dilution
7.1.5 Hydrolysis of sucrose and α-glucans
7.1.6 Carrez clarification (optional, use in case of difficulties passing sample through SPE)
17 7.1.7 Removal of monosaccharides
7.1.8 Hydrolysis of fructans
7.2 Chromatographic conditions using column A (6.13.1)
18 7.3 Chromatographic conditions using columns B (6.13.2)
19 7.4 System suitability test
7.5 Calibration
8 Calculation
21 9 Reporting
10 Precision data
10.1 General
10.2 Repeatability
10.3 Reproducibility
22 11 Test report
23 Annex A (informative) Example chromatograms and calibration curves
26 Annex B (informative) Precision data
27 Annex C (informative) Check test enzyme mixture of sucrase, β-amylase, pullulanase and maltase
30 Bibliography

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BS EN ISO 22579:2021
$142.49