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BS EN ISO 6579-1:2017

BS EN ISO 6579-1:2017

$198.66

Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella – Detection of Salmonella spp.

Published By Publication Date Number of Pages
BSI 2017 62
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PDF Catalog

PDF Pages PDF Title
7 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
4.1 General
4.2 Pre-enrichment in non-selective liquid medium
4.3 Enrichment in/on selective media
4.4 Plating out on selective solid media
13 4.5 Confirmation
5 Culture media, reagents, and antisera
6 Equipment and consumables
14 7 Sampling
8 Preparation of test sample
9 Procedure (see diagrams in Annex A)
9.1 Test portion and initial suspension
9.2 Non-selective pre-enrichment
15 9.3 Selective enrichment
9.3.1 General
9.3.2 Procedure for food, animal feed samples, and environmental samples from the food production area
9.3.3 Procedure for samples from the primary production stage
16 9.4 Plating out
9.4.1 General
9.4.2 Procedure for food, animal feed samples, and environmental samples from the food production area
9.4.3 Procedure for samples from the primary production stage
17 9.5 Confirmation
9.5.1 General
9.5.2 Selection of colonies for confirmation
18 9.5.3 Biochemical testing
21 9.5.4 Serological testing
9.5.5 Interpretation of biochemical and serological reactions
22 9.5.6 Serotyping
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory studies
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
23 12 Test report
24 Annex A (normative) Diagrams of the procedures
27 Annex B (normative) Culture media and reagents
42 Annex C (informative) Method validation studies and performance characteristics
48 Annex D (normative) Detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi
53 Annex E (informative) Examples of selective plating-out media
58 Bibliography

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BS EN ISO 6579-1:2017
$198.66