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BS EN ISO 6579-1:2017+A1:2020

BS EN ISO 6579-1:2017+A1:2020

$198.66

Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella – Detection of Salmonella spp.

Published By Publication Date Number of Pages
BSI 2020 62
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This document specifies a horizontal method for the detection of Salmonella. It is applicable to the following:

  • products intended for human consumption and the feeding of animals;

  • environmental samples in the area of food production and food handling;

  • samples from the primary production stage such as animal faeces, dust, and swabs.

With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.

The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
Anchor 2
European foreword to amendment A1
7 Foreword
9 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
12 4 Principle
4.1 General
4.2 Pre-enrichment in non-selective liquid medium
4.3 Enrichment in/on selective media
4.4 Plating out on selective solid media
13 4.5 Confirmation
5 Culture media, reagents, and antisera
6 Equipment and consumables
14 7 Sampling
8 Preparation of test sample
9 Procedure (see diagrams in Annex A)
9.1 Test portion and initial suspension
9.2 Non-selective pre-enrichment
15 9.3 Selective enrichment
9.3.1 General
9.3.2 Procedure for food, animal feed samples, and environmental samples from the food production area
9.3.3 Procedure for samples from the primary production stage
16 9.4 Plating out
9.4.1 General
9.4.2 Procedure for food, animal feed samples, and environmental samples from the food production area
9.4.3 Procedure for samples from the primary production stage
17 9.5 Confirmation
9.5.1 General
9.5.2 Selection of colonies for confirmation
18 9.5.3 Biochemical testing
21 9.5.4 Serological testing
9.5.5 Interpretation of biochemical and serological reactions
22 9.5.6 Serotyping
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory studies
11.2 Sensitivity
11.3 Specificity
11.4 LOD50
23 12 Test report
24 Annex A (normative) Diagrams of the procedures
27 Annex B (normative) Culture media and reagents
42 Annex C (informative) Method validation studies and performance characteristics
48 Annex D (informative) Detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi
53 Annex E (informative) Examples of selective plating-out media
58 Bibliography

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BS EN ISO 6579-1:2017+A1:2020
$198.66