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BS EN ISO 6888-2:2021

BS EN ISO 6888-2:2021

$142.49

Microbiology of the food chain. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Method using rabbit plasma fibrinogen agar medium

Published By Publication Date Number of Pages
BSI 2021 24
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This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]).

This document is applicable to:

  • products intended for human consumption;

  • products intended for animal feeding;

  • environmental samples in the area of food and feed production and handling;

  • samples from the primary production stage.

This horizontal method was originally developed for the examination of all samples belonging to the food chain.

Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.

Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:

  • staphylococci forming atypical colonies on a Baird-Parker agar medium;

— background flora that can obscure the colonies being sought.

Nevertheless, both ISO 6888-1 and this document are given equivalent status.

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
8 Introduction
9 1 Scope
10 2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Incubation
11 4.3 Enumeration
5 Culture media and reagents
6 Equipment and consumables
6.1 Apparatus for dry sterilization (oven) and wet sterilization (autoclave).
12 7 Sampling
8 Preparation of the test sample
9 Procedure (see Figure A.1)
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
13 9.3 Counting of colonies
9.3.1 General description of colonies growing on RPFA medium
9.3.2 Colony counting procedure
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
14 11.3 Reproducibility limit
15 12 Test report
13 Quality assurance
16 Annex A (normative) Flow diagram of the procedure
17 Annex B (normative) Culture media and reagents
20 Annex C (informative) Results of the interlaboratory study
22 Bibliography

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BS EN ISO 6888-2:2021
$142.49