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BS ISO 18184:2019 – TC:2020 Edition

$246.62

Tracked Changes. Textiles. Determination of antiviral activity of textile products

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BSI 2020 0
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This document specifies testing methods for the determination of the antiviral activity of the textile products against specified viruses. Due to the individual sensitivities, the results of one test virus cannot be transposed to other viruses.

The textile products include woven and knitted fabrics, fibres, yarns, braids, etc.

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PDF Pages PDF Title
3 National foreword
Compliance with a British Standard cannot confer immunity from legal obligations.
Amendments/corrigenda issued since publication
4 Textiles — Determination of antiviral
activity of textile products
5 COPYRIGHT PROTECTED DOCUMENT
8 Foreword
9 Introduction
10 Textiles — Determination of antiviral activity of textile products
1 Scope
2 Normative references
3 Terms and definitions
3.1
11 3.2
3.3
3.4
3.5
reference cloth control fabric
3.6
3.7
3.8
3.9
12 3.10
3.11
3.12
3.13
3.14
4 Principle
5 Virus and host cell
6 Warning
13 7 Apparatus
7.6 Washing machine.
14 Figure 1 — 96 wells microplate for TCID50 method
Figure 2 — 6 wells plastic plate for plaque assay
15 Figure 3 — Flask for cell culture use
7.26 Centrifuge tube.
8 Sterilization of apparatus
16 9 Reagent and medium
9.3 7,5 % sodium bicarbonate solution.
9.4 Formalin solution.
9.5 Methylene blue solution, use for the cells dyeing.
9.4 Formaldehyde solution.
9.5 Methylene blue solution.
17 9.6 Inactivated fetal bovine serum (FBS)
9.7 Growth medium, used for cell culture.
9.8 Maintenance medium, used for cell culture.
18 9.9 Double concentration of the maintenance medium (9.8).
9.10 0,01 mol/l phosphate buffered saline PBS (-).
9.11 Trypsin derived from beef pancreas and PBS (-) solution.
19 9.12 Trypsin EDTA solution.
9.13 DEAE-dextran solution.
9.14 Agar medium, used for the plaque assay. preparation.
20 9.14.1 A liquid.Liquid A.
9.14.2 Liquid B liquid.
21 9.16 Maintenance medium, used for cell culture and for TCID50 method.Maintenance mediumwith Trypsin.
10 Preparation
10.1 Restoration of host cell from cryopreservation
22 10.2 Subculture of host cell
10.3 Cell culture for the infectious virus titre assay
23 10.4 Preparation for test virus
10.4.1 General
10.4.2 Influenza virus
24 10.4.3 Feline calicivirus
25 10.4.4 Infectivity titre of the test viruses
10.4.4.1 Preparation for series of the dilution for the virus suspension
10.4.4.12 Infectivity measurement
10.4.4.2 Determination of the infectious titre
10.5 Preparation for test specimen
10.5.1 Control fabric
26 10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test
10.6.1 General
10.6.2 Verification of cytotoxic effect
10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
27 10.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure
11.1 Preparation of specimen
11.2 Inoculation of virus to the specimens
11.2 Deposit of virus to the specimens
11.3 Contacting time
28 11.4 Wash-out of virus immediately after inoculationdeposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
29 13 Infective titre measurement
13 Determination of the infectious titre
13.1 Plaque assay
14 Calculation of infectivity titre
14.2.1 The Behrens and Karber method
30 14.2.2 Example of calculation
31 Figure 4 — Example of TCID50 method
14.3 Test result
14.3.1 Verification of this test
32 14.3.2 Calculation of antiviral activity value
15 Test report
33 (normativeinformative)
Table A.1 — Viruses, host cells and media used for this standarddocument
34 Annex B
(normative)
B.1 Test procedure
35 B.2 Determination of PFU
B.2.1 General
Table B.2 — Interpretation of the data
37 Annex C
(normative)
C.1 Test procedure
C.1.1 Pick up 96 wells microplate with the monolayer cells grown in the each well of Clause 10.3 and observe by a microscope of a confluent state of the grown cells. After confirmation, drain extra growth medium from the plate.Observe at the microscop…
38 D.2 Composition of EMEM
Table D.1 — Composition of EMEM
39 Table D.2 — The Composition of RPMI 1640
40 Annex E
(informative)
E.1 General
Table E.1 — Polio virus strain and host cell
41 F.1E.1 General
F.2 Scope
E.2 Overview
F.3.1 SPF embryonated hen’s eggs
E.3 Specific pathogen free (SPF) embryonated hen’s eggs
F.4E.4 Preparation of specimen
F.5E.5 Preparation of the embryonated hen’s eggs
F.6E.6 Preparation of virus
42 F.6.1E.6.1 Virus cultivation
F.6.2E.6.2 Preparation of 0,5 % chicken red blood cell suspension
F.7E.7 Test procedure
F.7.1E.7.1 General
43 Figure F.1Figure E.1— General process image of embryonated eggs method
F.7.3E.7.3 Inoculation of the reacted virus suspension to 10 day old embryonated hen’s eggs
F.7.4E.7.4 Virus assay
F.7.5E.7.5 Calculation of the virus titre
44 Table F.1E.1 — Example of the EID test
46 Annex F
(informative)
Tabelle GTable F.1 — Antiviral performance standard
47 Table H.1 — Sample details for Round robin test
Table G.1 — Sample details for interlaboratory test
H.3G.3 Test condition
H.4G.4 Test result
H.4.1G.4.1 Test result of a testing house
48 Table H.2G.2— Test result of a testing house
H.4.2G.4.2 Test result of a laboratory
Table H.3G.3— Test result of a laboratory
49 Annex IH
(informative)
I.1H.1 Participants
I.2H.2 Sample
Table I.1H.1— Sample details for Round robininterlaboratory test
50 I.4H.4 Test result
I.4.1H.4.1 Sample A
Table I.2Table H.2— Round robinInterlaboratory test result for sample A
Table I.3H.3— Round robinInterlaboratory test result for sample B
51 I.4.3H.4.3 Sample C
Table I.4H.4 — Round robinInterlaboratory test result for sample C
Table I.5H.5— Summary of Antiviralantiviral activity
52 Bibliography
54 National foreword
59 Foreword
60 Introduction
61 1 Scope
2 Normative references
3 Terms and definitions
63 4 Principle
5 Virus and host cell
6 Warning
7 Apparatus
66 8 Sterilization of apparatus
9 Reagent and medium
67 9.4 Formaldehyde solution.
9.6 Inactivated fetal bovine serum (FBS)
71 10 Preparation
10.1 Restoration of host cell from cryopreservation
72 10.2 Subculture of host cell
73 10.3 Cell culture for the infectious virus titre assay
10.4 Preparation for test virus
10.4.1 General
10.4.2 Influenza virus
74 10.4.3 Feline calicivirus
75 10.4.4 Infectivity titre of the test viruses
76 10.5 Preparation for test specimen
10.5.1 Control fabric
10.5.2 Preparation of test specimens
10.5.3 Sterilization of specimens
10.6 Control test
10.6.1 General
77 10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity
11 Test procedure
11.1 Preparation of specimen
11.2 Deposit of virus to the specimens
11.3 Contacting time
78 11.4 Wash-out of virus immediately after deposit
11.5 Wash-out of virus after contacting time
12 Preparation of the series of the dilution for the virus suspension
13 Determination of the infectious titre
13.1 Plaque assay
79 13.2 TCID50 method
14 Calculation of infectivity titre
14.1 Plaque assay
14.2 TCID50 method
14.2.1 Behrens and Karber method
80 14.2.2 Example of calculation
81 14.3 Test result
14.3.1 Verification of this test
82 14.3.2 Calculation of antiviral activity value
15 Test report
83 Annex A (informative) Virus strains and host cells
84 Annex B (normative) Infectivity titre test: Plaque assay
86 Annex C (normative) Infectivity titre test: TCID50 method
87 Annex D (informative) Composition of media
90 Annex E (informative) Testing method using specific pathogen free (SPF) embryonated hen’s eggs
95 Annex F (informative) Antiviral efficacy — Antiviral performance of the products
96 Annex G (informative) Interlaboratory test result (1)
98 Annex H (informative) Interlaboratory test result (2)
101 Bibliography
BS ISO 18184:2019 - TC
$246.62