BS ISO 18184:2019 – TC:2020 Edition
$246.62
Tracked Changes. Textiles. Determination of antiviral activity of textile products
Published By | Publication Date | Number of Pages |
BSI | 2020 | 0 |
This document specifies testing methods for the determination of the antiviral activity of the textile products against specified viruses. Due to the individual sensitivities, the results of one test virus cannot be transposed to other viruses.
The textile products include woven and knitted fabrics, fibres, yarns, braids, etc.
PDF Catalog
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3 | National foreword Compliance with a British Standard cannot confer immunity from legal obligations. Amendments/corrigenda issued since publication |
4 | Textiles — Determination of antiviral activity of textile products |
5 | COPYRIGHT PROTECTED DOCUMENT |
8 | Foreword |
9 | Introduction |
10 | Textiles — Determination of antiviral activity of textile products 1 Scope 2 Normative references 3 Terms and definitions 3.1 |
11 | 3.2 3.3 3.4 3.5 reference cloth control fabric 3.6 3.7 3.8 3.9 |
12 | 3.10 3.11 3.12 3.13 3.14 4 Principle 5 Virus and host cell 6 Warning |
13 | 7 Apparatus 7.6 Washing machine. |
14 | Figure 1 — 96 wells microplate for TCID50 method Figure 2 — 6 wells plastic plate for plaque assay |
15 | Figure 3 — Flask for cell culture use 7.26 Centrifuge tube. 8 Sterilization of apparatus |
16 | 9 Reagent and medium 9.3 7,5 % sodium bicarbonate solution. 9.4 Formalin solution. 9.5 Methylene blue solution, use for the cells dyeing. 9.4 Formaldehyde solution. 9.5 Methylene blue solution. |
17 | 9.6 Inactivated fetal bovine serum (FBS) 9.7 Growth medium, used for cell culture. 9.8 Maintenance medium, used for cell culture. |
18 | 9.9 Double concentration of the maintenance medium (9.8). 9.10 0,01 mol/l phosphate buffered saline PBS (-). 9.11 Trypsin derived from beef pancreas and PBS (-) solution. |
19 | 9.12 Trypsin EDTA solution. 9.13 DEAE-dextran solution. 9.14 Agar medium, used for the plaque assay. preparation. |
20 | 9.14.1 A liquid.Liquid A. 9.14.2 Liquid B liquid. |
21 | 9.16 Maintenance medium, used for cell culture and for TCID50 method.Maintenance mediumwith Trypsin. 10 Preparation 10.1 Restoration of host cell from cryopreservation |
22 | 10.2 Subculture of host cell 10.3 Cell culture for the infectious virus titre assay |
23 | 10.4 Preparation for test virus 10.4.1 General 10.4.2 Influenza virus |
24 | 10.4.3 Feline calicivirus |
25 | 10.4.4 Infectivity titre of the test viruses 10.4.4.1 Preparation for series of the dilution for the virus suspension 10.4.4.12 Infectivity measurement 10.4.4.2 Determination of the infectious titre 10.5 Preparation for test specimen 10.5.1 Control fabric |
26 | 10.5.2 Preparation of test specimens 10.5.3 Sterilization of specimens 10.6 Control test 10.6.1 General 10.6.2 Verification of cytotoxic effect 10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity |
27 | 10.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity 11 Test procedure 11.1 Preparation of specimen 11.2 Inoculation of virus to the specimens 11.2 Deposit of virus to the specimens 11.3 Contacting time |
28 | 11.4 Wash-out of virus immediately after inoculationdeposit 11.5 Wash-out of virus after contacting time 12 Preparation of the series of the dilution for the virus suspension |
29 | 13 Infective titre measurement 13 Determination of the infectious titre 13.1 Plaque assay 14 Calculation of infectivity titre 14.2.1 The Behrens and Karber method |
30 | 14.2.2 Example of calculation |
31 | Figure 4 — Example of TCID50 method 14.3 Test result 14.3.1 Verification of this test |
32 | 14.3.2 Calculation of antiviral activity value 15 Test report |
33 | (normativeinformative) Table A.1 — Viruses, host cells and media used for this standarddocument |
34 | Annex B (normative) B.1 Test procedure |
35 | B.2 Determination of PFU B.2.1 General Table B.2 — Interpretation of the data |
37 | Annex C (normative) C.1 Test procedure C.1.1 Pick up 96 wells microplate with the monolayer cells grown in the each well of Clause 10.3 and observe by a microscope of a confluent state of the grown cells. After confirmation, drain extra growth medium from the plate.Observe at the microscop… |
38 | D.2 Composition of EMEM Table D.1 — Composition of EMEM |
39 | Table D.2 — The Composition of RPMI 1640 |
40 | Annex E (informative) E.1 General Table E.1 — Polio virus strain and host cell |
41 | F.1E.1 General F.2 Scope E.2 Overview F.3.1 SPF embryonated hen’s eggs E.3 Specific pathogen free (SPF) embryonated hen’s eggs F.4E.4 Preparation of specimen F.5E.5 Preparation of the embryonated hen’s eggs F.6E.6 Preparation of virus |
42 | F.6.1E.6.1 Virus cultivation F.6.2E.6.2 Preparation of 0,5 % chicken red blood cell suspension F.7E.7 Test procedure F.7.1E.7.1 General |
43 | Figure F.1Figure E.1— General process image of embryonated eggs method F.7.3E.7.3 Inoculation of the reacted virus suspension to 10 day old embryonated hen’s eggs F.7.4E.7.4 Virus assay F.7.5E.7.5 Calculation of the virus titre |
44 | Table F.1E.1 — Example of the EID test |
46 | Annex F (informative) Tabelle GTable F.1 — Antiviral performance standard |
47 | Table H.1 — Sample details for Round robin test Table G.1 — Sample details for interlaboratory test H.3G.3 Test condition H.4G.4 Test result H.4.1G.4.1 Test result of a testing house |
48 | Table H.2G.2— Test result of a testing house H.4.2G.4.2 Test result of a laboratory Table H.3G.3— Test result of a laboratory |
49 | Annex IH (informative) I.1H.1 Participants I.2H.2 Sample Table I.1H.1— Sample details for Round robininterlaboratory test |
50 | I.4H.4 Test result I.4.1H.4.1 Sample A Table I.2Table H.2— Round robinInterlaboratory test result for sample A Table I.3H.3— Round robinInterlaboratory test result for sample B |
51 | I.4.3H.4.3 Sample C Table I.4H.4 — Round robinInterlaboratory test result for sample C Table I.5H.5— Summary of Antiviralantiviral activity |
52 | Bibliography |
54 | National foreword |
59 | Foreword |
60 | Introduction |
61 | 1 Scope 2 Normative references 3 Terms and definitions |
63 | 4 Principle 5 Virus and host cell 6 Warning 7 Apparatus |
66 | 8 Sterilization of apparatus 9 Reagent and medium |
67 | 9.4 Formaldehyde solution. 9.6 Inactivated fetal bovine serum (FBS) |
71 | 10 Preparation 10.1 Restoration of host cell from cryopreservation |
72 | 10.2 Subculture of host cell |
73 | 10.3 Cell culture for the infectious virus titre assay 10.4 Preparation for test virus 10.4.1 General 10.4.2 Influenza virus |
74 | 10.4.3 Feline calicivirus |
75 | 10.4.4 Infectivity titre of the test viruses |
76 | 10.5 Preparation for test specimen 10.5.1 Control fabric 10.5.2 Preparation of test specimens 10.5.3 Sterilization of specimens 10.6 Control test 10.6.1 General |
77 | 10.6.2 Verification of cytotoxicity by cell sensitivity to virus and the inactivation of antiviral activity 11 Test procedure 11.1 Preparation of specimen 11.2 Deposit of virus to the specimens 11.3 Contacting time |
78 | 11.4 Wash-out of virus immediately after deposit 11.5 Wash-out of virus after contacting time 12 Preparation of the series of the dilution for the virus suspension 13 Determination of the infectious titre 13.1 Plaque assay |
79 | 13.2 TCID50 method 14 Calculation of infectivity titre 14.1 Plaque assay 14.2 TCID50 method 14.2.1 Behrens and Karber method |
80 | 14.2.2 Example of calculation |
81 | 14.3 Test result 14.3.1 Verification of this test |
82 | 14.3.2 Calculation of antiviral activity value 15 Test report |
83 | Annex A (informative) Virus strains and host cells |
84 | Annex B (normative) Infectivity titre test: Plaque assay |
86 | Annex C (normative) Infectivity titre test: TCID50 method |
87 | Annex D (informative) Composition of media |
90 | Annex E (informative) Testing method using specific pathogen free (SPF) embryonated hen’s eggs |
95 | Annex F (informative) Antiviral efficacy — Antiviral performance of the products |
96 | Annex G (informative) Interlaboratory test result (1) |
98 | Annex H (informative) Interlaboratory test result (2) |
101 | Bibliography |