BS ISO 20814:2019
$142.49
Nanotechnologies. Testing the photocatalytic activity of nanoparticles for NADH oxidation
| Published By | Publication Date | Number of Pages |
| BSI | 2019 | 30 |
This document specifies a method for the measurement of the photocatalytic activity (PCA) of nanoparticles (NPs), suspended in an aqueous environment in physiologically relevant conditions, by measuring the ultraviolet (UV)-induced nicotine adenine dinucleotide hydrate (NADH) oxidation.
The measurement is intended to assess the potential for the photo-toxicity of nanomaterials. The method is also applicable to NP aggregates and agglomerates.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 6 | Foreword |
| 7 | Introduction |
| 8 | 1 Scope 2 Normative references 3 Terms, definitions, symbols and abbreviated terms 3.1 Terms and definitions |
| 9 | 3.2 Symbols and abbreviated terms |
| 10 | 4 Description of the test method 5 Reagents and apparatus 5.1 Reagents |
| 11 | 5.2 Apparatus 6 Measurement procedure 6.1 Measurement of NP suspension basic properties 6.1.1 UV-Vis absorption spectrum measurement |
| 12 | 6.1.2 NP suspension stability measurement 6.2 UV trans-illuminator light intensity calibration based on 2NB actinometry |
| 13 | 6.3 Measurement of NADH solution fluorescence intensity 6.3.1 NADH photo-oxidation rate measurement at various NP concentrations |
| 14 | 6.3.2 Calculation of NADH photo-oxidation rate at various NP concentrations |
| 15 | 7 Test report 7.1 Information |
| 16 | 7.2 Report data format 7.2.1 Correction factors C(i,j) obtained by actinometry (see 7.2) with Ī»(max,TI) 7.2.2 Calibrated slope of NADH fluorescence decrease |
| 17 | 7.2.3 Plot of kapp versus NP concentration 7.2.4 NADH equivalent specific PCA 8 Precision 8.1 Repeatability 8.2 Reproducibility |
| 18 | Annex A (normative) Schematic diagram of 96-well positioning block |
| 19 | Annex B (informative) Sample calibration of UV trans-illuminator light intensity |
| 24 | Annex C (informative) Interlaboratory comparison study of TiO2 NP PCA |
| 29 | Bibliography |
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