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BS ISO 21160:2018

BS ISO 21160:2018

$142.49

Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2018 26
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This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD.

This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
4 Principle
10 5 Apparatus
11 6 Reagents
12 7 Preparation
7.1 Preparation of glassware
7.2 Preparation of solutions
7.3 Preparation of standards
7.3.1 HPLC calibration standards and working solutions
13 7.3.2 Carbonyl working standards
8 Sampling
9 Tobacco product preparation
10 Sample generation — Smoking of cigarettes
10.1 General
10.2 Smoking machine setup
14 10.3 Smoking
10.3.1 General
10.3.2 Linear smoking
10.3.3 Rotary smoking
15 11 Sample analysis
11.1 Preparation of mainstream smoke extract solution
11.2 Reversed phase high performance liquid chromatography
11.2.1 Chromatographic conditions
11.2.2 Mobile phase reagents
11.2.3 HPLC separation conditions
16 11.3 Calculation
11.3.1 Calibration curve
11.3.2 Sample quantification
17 12 Repeatability and reproducibility
12.1 General
12.2 Results from the 2012 collaborative study
20 13 Test report
21 Annex A (informative) Recrystallization of 2,4-dinitrophenylhydrazine
22 Annex B (normative) Example of calibration standards preparation
23 Annex C (informative) HPLC chromatogram of a typical combined carbonyl calibration standard
24 Annex D (informative) HPLC chromatogram of carbonyls in DNPH extract of mainstream cigarette smoke
25 Bibliography

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BS ISO 21160:2018
$142.49