BS ISO 21703:2019
$142.49
Surface active agents. Microbiology. Microbiological test methods for liquid hand dishwashing
| Published By | Publication Date | Number of Pages |
| BSI | 2019 | 28 |
This document provides microbiological test methods for enumeration and detection of aerobic mesophilic bacteria, detection of Escherichia coli and Pseudomonas aeruginosa in liquid hand dishwashing.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | National foreword |
| 7 | Foreword |
| 9 | 1 Scope 2 Normative references 3 Terms and definitions |
| 10 | 4 Principle 5 Diluents and culture media 5.1 General 5.2 Neutralizing diluent and diluents 5.2.1 General |
| 11 | 5.2.2 Neutralizing diluent: Fluid casein digest — soy lecithin — polysorbate 20 medium (SCDLP 20 broth) 5.2.3 Diluent for the bacterial suspension (Tryptone sodium chloride solution) 5.3 Culture medium for counting 5.3.1 General 5.3.2 Soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA) |
| 12 | 5.3.3 Other medium for counting 5.4 Culture medium for detection 5.4.1 General 5.4.2 Enrichment broth: Eugon LT 100 broth |
| 13 | 5.5 Culture media for isolation and identification 5.5.1 MacConkey agar medium |
| 14 | 5.5.2 Levine eosin-methylene blue agar medium (EMB agar medium) 5.5.3 Cetrimide agar medium |
| 15 | 5.5.4 Pseudomonas agar medium for detection of pyocyanin (Pseudomonas agar P) 6 Apparatus and glassware 7 Strains of microorganisms 8 Handling of the product and laboratory samples |
| 16 | 9 Procedure 9.1 General recommendation 9.2 Preparation of the initial suspension 9.3 Enumeration methods 9.3.1 Dilutions for enumeration 9.3.2 Plate-count methods |
| 18 | 10 Method for detection and identification 10.1 Enrichment 10.2 Isolation |
| 19 | 10.3 Detection of aerobic mesophilic bacteria 10.4 Detection and Identification of Escherichia coli 10.4.1 General 10.4.2 Gram’s stain 10.4.3 Culture on Levine eosin-methylene blue agar medium (EMB agar medium) 10.4.4 Expression of results 10.5 Detection and identification of Pseudomonas aeruginosa 10.5.1 General |
| 20 | 10.5.2 Gram’s stain 10.5.3 Oxidase test 10.5.4 Culture on Pseudomonas agar medium for detection of pyocyanin 10.5.5 Expression of results 11 Neutralization of the antimicrobial properties of the product 11.1 General 11.2 Preparation of inoculum 11.3 Suitability of counting methods 11.3.1 Principle |
| 21 | 11.3.2 Suitability test of the pour-plate method 11.3.3 Suitability of the surface spread method 11.3.4 Suitability of the membrane filtration method 11.4 Suitability of the detection method 11.4.1 Procedure |
| 22 | 11.4.2 Interpretation 12 Test report |
| 23 | Annex A (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids |
| 24 | Annex B (informative) Other culture media |
| 26 | Bibliography |
