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BSI PD CEN ISO/TS 15216-1:2013

BSI PD CEN ISO/TS 15216-1:2013

$167.15

Microbiology of food and animal feed. Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR – Method for quantification

Published By Publication Date Number of Pages
BSI 2013 44
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PDF Catalog

PDF Pages PDF Title
6 Foreword
9 Introduction
11 Section sec_1
Section sec_2
Section sec_3
Section sec_3.1
Section sec_3.2
Section sec_3.3
1 Scope
2 Normative references
3 Terms and definitions
12 Section sec_3.4
Section sec_3.5
Section sec_3.6
Section sec_3.7
Section sec_3.8
Section sec_3.9
Section sec_3.10
Section sec_3.11
Section sec_3.12
13 Section sec_3.13
Section sec_3.14
Section sec_3.15
Section sec_3.16
Section sec_3.17
Section sec_3.18
Section sec_4
Section sec_4.1
Section sec_4.2
4 Principle
4.1 Virus extraction
4.2 RNA extraction
14 Section sec_4.3
Section sec_4.4
Section sec_4.4.1
Section sec_4.4.2
Section sec_4.4.3
4.3 Real-time reverse transcription polymerase chain reaction (real time RT-PCR)
4.4 Control materials
15 Section sec_4.5
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.2.1
Section sec_5.2.2
Section sec_5.2.3
Section sec_5.2.4
Section sec_5.2.5
Section sec_5.2.6
Section sec_5.2.7
Section sec_5.2.8
Section sec_5.2.9
Section sec_5.2.10
Section sec_5.2.11
Section sec_5.2.12
Section sec_5.2.13
Section sec_5.2.14
Section sec_5.2.15
Section sec_5.2.16
4.5 Test results
5 Reagents
5.1 General
5.2 Reagents used as supplied
16 Section sec_5.2.17
Section sec_5.2.18
Section sec_5.2.19
Section sec_5.3
Section sec_5.3.1
Section sec_5.3.2
Section sec_5.3.3
Section sec_5.3.4
Section sec_5.3.5
Section sec_5.3.6
Section sec_5.3.7
Section sec_5.3.8
5.3 Prepared reagents
17 Section sec_5.3.9
Section sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.3
Section sec_6.4
Section sec_6.5
Section sec_6.6
Section sec_6.7
Section sec_6.8
Section sec_6.9
Section sec_6.10
Section sec_6.11
Section sec_6.12
Section sec_6.13
6 Apparatus and materials
18 Section sec_6.14
Section sec_6.15
Section sec_6.16
Section sec_6.17
Section sec_6.18
Section sec_6.19
Section sec_6.20
Section sec_6.21
Section sec_6.22
Section sec_6.23
Section sec_6.24
Section sec_6.25
Section sec_6.26
Section sec_6.27
Section sec_6.28
Section sec_7
Section sec_8
Section sec_8.1
7 Sampling
8 Procedure
8.1 General laboratory requirements
19 Section sec_8.2
Section sec_8.2.1
Section sec_8.2.2
Section sec_8.2.3
Section sec_8.2.4
8.2 Virus extraction
20 Section sec_8.2.5
Section sec_8.2.6
21 Section sec_8.3
Section sec_8.4
Section sec_8.4.1
Section sec_8.4.2
Section sec_8.4.2.1
8.3 RNA extraction
8.4 Real-time RT-PCR
22 Section sec_8.4.2.2
Section sec_8.4.2.3
23 Section sec_8.4.2.4
Section sec_9
Section sec_9.1
Section sec_9.2
Section sec_9.3
9 Interpretation of results
9.1 General
9.2 Construction of standard curves
9.3 Calculation of amplification efficiency
24 Section sec_9.4
Section sec_9.5
9.4 Calculation of extraction efficiency
9.5 Sample quantification
25 Section sec_9.6
Section sec_10
Section sec_11
9.6 Theoretical limit of detection
10 Expression of results
11 Test report
27 Annex sec_A
Annex A
(normative)

Diagram of procedure

28 Annex sec_B
Table tab_B.1
Table tab_B.2
Annex B
(informative)

Real-time RT-PCR mastermixes and cycling parameters

29 Annex sec_C
Annex sec_C.1
Annex sec_C.2
Annex C
(informative)

Real-time RT-PCR primers and hydrolysis probes for the detection of HAV, norovirus GI and GII and mengo virus (process control)

30 Annex sec_C.3
Annex sec_C.4
31 Annex sec_D
Annex sec_D.1
Annex sec_D.2
Annex sec_D.2.1
Annex sec_D.2.2
Annex sec_D.3
Annex D
(informative)

Growth of mengo virus strain MC0 for use as a process control

32 Annex sec_E
Annex sec_E.1
Annex sec_E.1.1
Annex sec_E.1.2
Annex sec_E.2
Annex sec_E.2.1
Annex sec_E.2.2
Annex sec_E.2.3
Annex sec_E.2.4
Annex sec_E.3
Annex E
(informative)

RNA extraction using the BioMerieux NucliSens® system
Annex E
(informative)

RNA extraction using the BioMerieux NucliSens® system

34 Annex sec_F
Annex sec_F.1
Annex sec_F.1.1
Annex sec_F.1.2
Annex sec_F.2
Annex sec_F.2.1
Annex sec_F.2.2
Annex sec_F.3
Annex sec_F.3.1
Annex sec_F.3.2
Annex F
(normative)

Composition and preparation of reagents and buffers
Annex F
(normative)

Composition and preparation of reagents and buffers

35 Annex sec_F.4
Annex sec_F.4.1
Annex sec_F.4.2
Annex sec_F.5
Annex sec_F.5.1
Annex sec_F.5.2
36 Annex sec_G
Annex sec_G.1
Annex sec_G.2
Annex sec_G.2.1
Annex sec_G.2.2
Annex sec_G.2.3
Annex sec_G.2.4
Annex sec_G.2.5
Annex sec_G.2.6
Annex sec_G.2.7
Annex sec_G.2.8
Annex sec_G.3
Annex sec_G.4
Annex G
(informative)

Generation of double-stranded DNA (dsDNA) control stocks
Annex G
(informative)

Generation of double-stranded DNA (dsDNA) control stocks

37 Annex sec_G.5
38 Annex sec_H
Annex sec_H.1
Annex sec_H.2
Annex sec_H.2.1
Annex sec_H.2.2
Annex sec_H.2.3
Annex sec_H.2.4
Annex sec_H.2.5
Annex sec_H.2.6
Annex sec_H.2.7
Annex sec_H.2.8
Annex sec_H.3
Annex sec_H.4
Annex H
(informative)

Generation of external control RNA (EC RNA) stocks
Annex H
(informative)

Generation of external control RNA (EC RNA) stocks

39 Annex sec_H.5
Annex sec_H.6
40 Annex sec_I
Annex I
(informative)

Typical optical plate layout
Annex I
(informative)

Typical optical plate layout

41 Reference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
Reference ref_9
Reference ref_10
Reference ref_11
Reference ref_12
Bibliography
Bibliography

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BSI PD CEN ISO/TS 15216-1:2013
$167.15