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BSI PD CEN/TS 15634-4:2016

BSI PD CEN/TS 15634-4:2016

$102.76

Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR

Published By Publication Date Number of Pages
BSI 2016 18
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This Technical Specification describes a procedure for the qualitative detection of peanut ( Arachis hypogaea) in chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4], [5].

PDF Catalog

PDF Pages PDF Title
4 Contents Page
5 European foreword
6 1 Scope
2 Principle
3 Reagents
3.1 DNA extraction with CTAB
7 3.2 DNA purification by means of solid phase extraction
3.3 Real-time PCR reagents
4 Apparatus and equipment
8 4.1 DNA extraction
4.2 PCR
5 Procedure
5.1 General
5.2 Sample preparation
5.3 DNA extraction with CTAB
9 5.4 DNA purification by means of solid phase extraction
5.5 Measuring the mass concentration and purity of the extracted DNA
10 5.6 Real-time PCR
Table 1 — Reaction batch for real-time PCR
11 Table 2 — Temperature/time program when using a block cycler
6 Validation status and performance criteria
6.1 General
12 6.2 Detection
6.3 Reliability of the method
6.3.1 Setup of the interlaboratory study
6.3.2 Results
13 Table 3 — Overview data for the interlaboratory study
Table 4 — Number of PCR reactions showing false-negative or false-positive results
6.3.3 Specificity
7 Test report
15 Bibliography

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BSI PD CEN/TS 15634-4:2016
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