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BSI PD CEN/TS 17720:2022

BSI PD CEN/TS 17720:2022

$102.76

Plant biostimulants. Determination of Enterococcaceae

Published By Publication Date Number of Pages
BSI 2022 18
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This methodology has been developed to determine enterococci in biostimulants as a single microorganism component or in a mixture with other microorganisms. This document is not applicable to mineral fertilizers that are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) No 767/2009) [3].

PDF Catalog

PDF Pages PDF Title
2 undefined
8 1 Scope
2 Normative references
3 Terms and definitions
9 4 Principle
5 Diluents, selective media and test kit for phenotypic characterization
5.1 General
5.2 Diluent for initial suspension and for serial dilutions
5.3 Selective media
5.4 Phenotypic characterization and confirmation
10 6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
8.1 General
8.2 Liquid – water-based formulations
11 8.3 Liquid – oil-based (emulsifiable concentrate – EC) formulations
8.4 Solid – wettable powder (WP) formulations
8.5 Solid – water dispersible granules (WDG) formulations
8.6 Solid – pellets, granules, microgranules (slow release) formulations
8.7 Solid – substrate
9 Procedure
9.1 Inoculation and incubation of the plates
12 9.2 Evaluation of results
9.3 Confirmation step on BEA
9.4 Catalase test
10 Expression of results
11 Precision
11.1 General
11.2 Interlaboratory study
11.3 Repeatability
13 11.4 Reproducibility
12 Test report
14 Annex A (normative)Solutions, media
A.1 Diluents for initial suspension and serial dilutions
A.1.1 General
A.1.2 Phosphate buffered saline (PBS)
A.1.3 Peptone salt solution
A.1.4 Buffered peptone water (BPW)
15 A.2 Elective media
A.2.1 Slanetz Bartley agar
A.2.2 Bile Esculin Azide agar

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BSI PD CEN/TS 17720:2022
$102.76