ISO 18744:2016
$36.40
Microbiologie de la chaîne alimentaire — Recherche et dénombrement de Cryptospridium et Giardia dans les légumes verts frais à feuilles et les fruits à baies
| Published By | Publication Date | Number of Pages |
| ISO | 2016-04 | 30 |
ISO 18744:2016 spécifie une méthode applicable à la détection et au dénombrement des oocystes de Cryptosporidium et aux kystes de Giardia sur ou dans des produits alimentaires qui sont décrits ici comme étant des légumes verts frais à feuilles et des fruits à baies. Grâce à des contrôles appropriés, elle peut être également applicable à l'examen d'autres produits frais.
Les descriptions faites au microscope concernent les oocystes de Cryptosporidium spp. et les kystes de Giardia duodenalis dont la taille inclut les espèces (Cryptosporidium) ou assemblages (Giardia) connus pour être pathogènes pour l'homme.
Cette méthode n'inclut pas l'analyse moléculaire et n'est donc pas applicable à la détermination des espèces ou des génotypes/assemblages d'oocystes de Cryptosporidium et de kystes de Giardia. La méthode permettra de détecter toutes les espèces et tous les génotypes/assemblages connus pour être pathogènes pour l'homme ainsi que d'autres qui ne le sont pas. Pour mieux les identifier, des essais de typage moléculaire sont requis. Toutefois, ceux-ci ne peuvent pas être effectués de manière fiable si des contrôles de processus positifs ont été ajoutés aux échantillons, car le résultat des essais de typage moléculaire sera erroné.
Cette méthode ne permet pas de déterminer la viabilité ou l'infectivité des oocystes de Cryptosporidium et des kystes de Giardia qui peuvent être présents.
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