ISO/TS 21569-6:2016
$19.50
Méthodes horizontales d’analyse moléculaire de biomarqueurs — Méthodes d’analyse pour la détection des organismes génétiquement modifiés et des produits dérivés — Partie 6: Méthodes de criblage par PCR en temps réel pour la détection des séquences ADN cry1Ab/Ac et Pubi-cry
| Published By | Publication Date | Number of Pages |
| ISO | 2016-11 | 16 |
L'ISO/TS 21569-6:2016 spécifie une méthode de détection d'une séquence d'ADN du gène cry1Ab/Ac modifié et une méthode de détection de la séquence d'ADN de transition entre le promoteur de l'ubiquitine du maïs (Pubi) et le gène cry1Ab/Ac. Le gène cry1Ab/Ac modifié et le construit Pubi-cry sont fréquemment observés dans les plantes génétiquement modifiées contenant le gène Bt. Les deux méthodes sont basées sur une méthode par PCR en temps réel et peuvent être utilisées à des fins de criblage qualitatif. Pour l'identification et la quantification d'une plante génétiquement modifiée (événement) spécifique, une analyse complémentaire doit être effectuée.
L'ISO/TS 21569-6:2016 est applicable à l'analyse de l'ADN extrait de produits alimentaires. Il peut être également utilisé pour analyser l'ADN extrait d'autres produits tels que des aliments pour animaux et des semences. L'application de ces méthodes exige qu'une quantité adéquate d'ADN amplifiable soit extraite de la matrice étudiée.
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