{"id":338093,"date":"2024-10-19T23:39:04","date_gmt":"2024-10-19T23:39:04","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-128222014\/"},"modified":"2024-10-25T22:44:56","modified_gmt":"2024-10-25T22:44:56","slug":"bs-en-128222014","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-128222014\/","title":{"rendered":"BS EN 12822:2014"},"content":{"rendered":"

This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of \u03b1-, \u03b2-, \u03b3- and \u03b4-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of \u03b1-tocopherol in margarine and milk powder ranging from 9,89 mg\/100 g to 24,09 mg\/100 g. The second study was for the analysis of \u03b1-, \u03b2-, \u03b3- and \u03b4-tocopherol in milk powder and of \u03b1-, and \u03b2-tocopherol in oat powder ranging from 0,057 mg\/100 g (\u03b2-tocopherol) to 10,2 mg\/100 g (\u03b1-tocopherol).<\/p>\n

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NOTE The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].<\/p>\n<\/blockquote>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
5<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\nIntroduction <\/td>\n<\/tr>\n
7<\/td>\n1 Scope
2 Normative references
3 Principle
4 Reagents <\/td>\n<\/tr>\n
9<\/td>\nTable 1 \u2014 Examples for values and calculated \u03b5 <\/td>\n<\/tr>\n
10<\/td>\n5 Apparatus <\/td>\n<\/tr>\n
11<\/td>\n6 Procedure
6.1 Preparation of the test sample
6.2 Preparation of the sample test solution
6.2.1 Precautions
6.2.2 Oil and fat samples with low water content containing unesterified tocopherols
6.2.2.1 Oil and fat with low water content
6.2.2.2 Margarine and butter
6.2.3 Other samples
6.2.3.1 Saponification
Table 2 \u2014 Suitable ratios of reagents <\/td>\n<\/tr>\n
12<\/td>\n6.2.3.2 Extraction
6.2.3.3 Evaporation
6.2.3.4 Dilution
6.3 Identification <\/td>\n<\/tr>\n
13<\/td>\n6.4 Determination
7 Calculation
8 Precision
8.1 General <\/td>\n<\/tr>\n
14<\/td>\n8.2 Repeatability
8.3 Reproducibility
9 Test report <\/td>\n<\/tr>\n
16<\/td>\nAnnex A (informative) Examples of HPLC chromatograms
Figure A.1 \u2014 Example of a HPLC separation of \u03b1-, \u03b2-, \u03b3- and \u03b4-tocopherol from a margarine sample (CRM 122) <\/td>\n<\/tr>\n
17<\/td>\nFigure A.2 \u2014 Example of a HPLC separation of \u03b1-, \u03b2-, \u03b3- and \u03b4-tocopherol from a milk powder sample (CRM 421) <\/td>\n<\/tr>\n
18<\/td>\nAnnex B (informative) Precision data
Table B.1 \u2014 Precision data for margarine and milk powder <\/td>\n<\/tr>\n
19<\/td>\nTable B.2 \u2014 Precision data for milk powder
Table B.3 \u2014 Precision data for oat powder <\/td>\n<\/tr>\n
20<\/td>\nAnnex C (informative) Alternative HPLC systems
Table C.1 \u2014 Alternative HPLC conditions <\/td>\n<\/tr>\n
21<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Determination of vitamin E by high performance liquid chromatography. Measurement of \u03b1-, \u03b2-, \u03b3- and \u03b4-tocopherol<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2014<\/td>\n24<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338100,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338093","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338093","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338100"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338093"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338093"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}