{"id":338307,"date":"2024-10-19T23:40:15","date_gmt":"2024-10-19T23:40:15","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-ts-15634-42016\/"},"modified":"2024-10-25T22:46:18","modified_gmt":"2024-10-25T22:46:18","slug":"bsi-pd-cen-ts-15634-42016","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-ts-15634-42016\/","title":{"rendered":"BSI PD CEN\/TS 15634-4:2016"},"content":{"rendered":"

This Technical Specification describes a procedure for the qualitative detection of peanut ( Arachis hypogaea<\/i>) in chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4]<\/span>, [5]<\/span>.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nContents Page <\/td>\n<\/tr>\n
5<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\n1 Scope
2 Principle
3 Reagents
3.1 DNA extraction with CTAB <\/td>\n<\/tr>\n
7<\/td>\n3.2 DNA purification by means of solid phase extraction
3.3 Real-time PCR reagents
4 Apparatus and equipment <\/td>\n<\/tr>\n
8<\/td>\n4.1 DNA extraction
4.2 PCR
5 Procedure
5.1 General
5.2 Sample preparation
5.3 DNA extraction with CTAB <\/td>\n<\/tr>\n
9<\/td>\n5.4 DNA purification by means of solid phase extraction
5.5 Measuring the mass concentration and purity of the extracted DNA <\/td>\n<\/tr>\n
10<\/td>\n5.6 Real-time PCR
Table 1 \u2014 Reaction batch for real-time PCR <\/td>\n<\/tr>\n
11<\/td>\nTable 2 \u2014 Temperature\/time program when using a block cycler
6 Validation status and performance criteria
6.1 General <\/td>\n<\/tr>\n
12<\/td>\n6.2 Detection
6.3 Reliability of the method
6.3.1 Setup of the interlaboratory study
6.3.2 Results <\/td>\n<\/tr>\n
13<\/td>\nTable 3 \u2014 Overview data for the interlaboratory study
Table 4 \u2014 Number of PCR reactions showing false-negative or false-positive results
6.3.3 Specificity
7 Test report <\/td>\n<\/tr>\n
15<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Foodstuffs. Detection of food allergens by molecular biological methods – Peanut (Arachis hypogaea). Qualitative detection of a specific DNA sequence in chocolate by real-time PCR<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2016<\/td>\n18<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":338313,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-338307","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/338307","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/338313"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=338307"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=338307"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=338307"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}