{"id":338445,"date":"2024-10-19T23:40:59","date_gmt":"2024-10-19T23:40:59","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-211602018\/"},"modified":"2024-10-25T22:47:04","modified_gmt":"2024-10-25T22:47:04","slug":"bs-iso-211602018","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-211602018\/","title":{"rendered":"BS ISO 21160:2018"},"content":{"rendered":"
This document specifies a method for the determination of selected carbonyls (formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n<\/i>-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV\/DAD.<\/p>\n
This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg\/cigarette and 15 mg\/cigarette using reversed phase HPLC-UV\/DAD.<\/p>\n
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2<\/td>\n | National foreword <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions 4 Principle <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 5 Apparatus <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 6 Reagents <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 7 Preparation 7.1 Preparation of glassware 7.2 Preparation of solutions 7.3 Preparation of standards 7.3.1 HPLC calibration standards and working solutions <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 7.3.2 Carbonyl working standards 8 Sampling 9 Tobacco product preparation 10 Sample generation \u2014 Smoking of cigarettes 10.1 General 10.2 Smoking machine setup <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 10.3 Smoking 10.3.1 General 10.3.2 Linear smoking 10.3.3 Rotary smoking <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 11 Sample analysis 11.1 Preparation of mainstream smoke extract solution 11.2 Reversed phase high performance liquid chromatography 11.2.1 Chromatographic conditions 11.2.2 Mobile phase reagents 11.2.3 HPLC separation conditions <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 11.3 Calculation 11.3.1 Calibration curve 11.3.2 Sample quantification <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | 12 Repeatability and reproducibility 12.1 General 12.2 Results from the 2012 collaborative study <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | 13 Test report <\/td>\n<\/tr>\n | ||||||
21<\/td>\n | Annex A (informative) Recrystallization of 2,4-dinitrophenylhydrazine <\/td>\n<\/tr>\n | ||||||
22<\/td>\n | Annex B (normative) Example of calibration standards preparation <\/td>\n<\/tr>\n | ||||||
23<\/td>\n | Annex C (informative) HPLC chromatogram of a typical combined carbonyl calibration standard <\/td>\n<\/tr>\n | ||||||
24<\/td>\n | Annex D (informative) HPLC chromatogram of carbonyls in DNPH extract of mainstream cigarette smoke <\/td>\n<\/tr>\n | ||||||
25<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Cigarettes. Determination of selected carbonyls in the mainstream smoke of cigarettes. Method using high performance liquid chromatography<\/b><\/p>\n |