{"id":409160,"date":"2024-10-20T05:34:14","date_gmt":"2024-10-20T05:34:14","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-246512022\/"},"modified":"2024-10-26T10:12:13","modified_gmt":"2024-10-26T10:12:13","slug":"bs-iso-246512022","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-246512022\/","title":{"rendered":"BS ISO 24651:2022"},"content":{"rendered":"

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
8<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
13<\/td>\n4 Abbreviated terms and symbols <\/td>\n<\/tr>\n
15<\/td>\n5 General requirements
5.1 General
5.2 Personnel, facilities and equipment <\/td>\n<\/tr>\n
16<\/td>\n5.3 Reagents, consumables and other supplies
5.4 Management of information and data
6 Collection of bone marrow samples and associated data
6.1 Information about the bone marrow donor <\/td>\n<\/tr>\n
17<\/td>\n6.2 Anatomical collection site
6.3 Collection volume
6.4 Collection procedure
6.4.1 General <\/td>\n<\/tr>\n
18<\/td>\n6.4.2 Obtaining bone marrow by puncturing into the intramedullary canal
6.4.3 Obtaining bone marrow by aspirate
7 Transport of bone marrow samples or hBM-MSCs and associated data to the biobank
8 Reception and traceability of bone marrow or hBM-MSCs and associated data <\/td>\n<\/tr>\n
19<\/td>\n9 Isolation and expansion of hBM-MSCs
9.1 Processes
9.2 Unique identification
9.3 Testing for infectious agents
9.4 Isolation of hBM-MSCs from bone marrow samples obtained by puncturing into the intramedullary canal and primary culture <\/td>\n<\/tr>\n
20<\/td>\n9.5 Isolation of hBM-MSCs from bone marrow samples obtained by aspirate and primary culture
9.6 Subculture and limited expansion
10 Characterization of hBM-MSCs
10.1 General <\/td>\n<\/tr>\n
21<\/td>\n10.2 Viability
10.3 Morphology
10.4 Population doubling time and subculture\/passage
10.4.1 PDT <\/td>\n<\/tr>\n
22<\/td>\n10.4.2 Subculture\/passage
10.5 Cell population purity
10.6 In vitro self-renewal assessment
10.7 Proliferation <\/td>\n<\/tr>\n
23<\/td>\n10.8 Differentiation capability \u2014 In vitro multilineage differentiation
10.8.1 General
10.8.2 In vitro adipogenic differentiation
10.8.3 In vitro chondrogeneic differentiation <\/td>\n<\/tr>\n
24<\/td>\n10.8.4 In vitro osteogenic differentiation
10.9 Immunophenotyping by flow cytometry <\/td>\n<\/tr>\n
25<\/td>\n10.10 Paracrine secretion\/expression (protein-based assay of secretome)
10.11 Immunoregulation (modulation of immune cells) <\/td>\n<\/tr>\n
26<\/td>\n10.12 Microbial contamination
11 Quality control <\/td>\n<\/tr>\n
27<\/td>\n12 Storage <\/td>\n<\/tr>\n
28<\/td>\n13 Thawing
14 Disposal
15 Distribution of hBM-MSCs \u2014 Information for users <\/td>\n<\/tr>\n
29<\/td>\n16 Transport of hBM-MSCs
16.1 General <\/td>\n<\/tr>\n
30<\/td>\n16.2 hBM-MSCs frozen in ampoules or cryovials
16.3 Living hBM-MSC cultures <\/td>\n<\/tr>\n
31<\/td>\nAnnex A (informative) Preparation of human bone marrow mononuclear cells (hBM-MNCs) <\/td>\n<\/tr>\n
32<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Biotechnology. Biobanking. Requirements for human mesenchymal stromal cells derived from bone marrow<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2022<\/td>\n36<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":409169,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-409160","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/409160","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/409169"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=409160"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=409160"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=409160"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}