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BS EN 12822:2014

BS EN 12822:2014

$142.49

Foodstuffs. Determination of vitamin E by high performance liquid chromatography. Measurement of α-, β-, γ- and δ-tocopherol

Published By Publication Date Number of Pages
BSI 2014 24
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This European Standard specifies a method for the determination of vitamin E in foods by high performance liquid chromatography (HPLC). The determination of vitamin E content is carried out by measurement of α-, β-, γ- and δ-tocopherol. This method has been validated in two interlaboratory studies. The first study was for the analysis of α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g. The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder and of α-, and β-tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol) to 10,2 mg/100 g (α-tocopherol).

NOTE The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].

PDF Catalog

PDF Pages PDF Title
5 Foreword
6 Introduction
7 1 Scope
2 Normative references
3 Principle
4 Reagents
9 Table 1 — Examples for values and calculated ε
10 5 Apparatus
11 6 Procedure
6.1 Preparation of the test sample
6.2 Preparation of the sample test solution
6.2.1 Precautions
6.2.2 Oil and fat samples with low water content containing unesterified tocopherols
6.2.2.1 Oil and fat with low water content
6.2.2.2 Margarine and butter
6.2.3 Other samples
6.2.3.1 Saponification
Table 2 — Suitable ratios of reagents
12 6.2.3.2 Extraction
6.2.3.3 Evaporation
6.2.3.4 Dilution
6.3 Identification
13 6.4 Determination
7 Calculation
8 Precision
8.1 General
14 8.2 Repeatability
8.3 Reproducibility
9 Test report
16 Annex A (informative) Examples of HPLC chromatograms
Figure A.1 — Example of a HPLC separation of α-, β-, γ- and δ-tocopherol from a margarine sample (CRM 122)
17 Figure A.2 — Example of a HPLC separation of α-, β-, γ- and δ-tocopherol from a milk powder sample (CRM 421)
18 Annex B (informative) Precision data
Table B.1 — Precision data for margarine and milk powder
19 Table B.2 — Precision data for milk powder
Table B.3 — Precision data for oat powder
20 Annex C (informative) Alternative HPLC systems
Table C.1 — Alternative HPLC conditions
21 Bibliography

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BS EN 12822:2014
$142.49