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BS EN ISO 11290-2:1998+A1:2004:2009 Edition

BS EN ISO 11290-2:1998+A1:2004:2009 Edition

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Microbiology of food and animal feeding Stuffs. Horizontal method for the detection and enumeration of Listeria monocytogenes – Enumeration method

Published By Publication Date Number of Pages
BSI 2009 28
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PDF Catalog

PDF Pages PDF Title
1 BRITISH STANDARD
2 National foreword
4 Foreword
Foreword to amendment A1
8 Introduction
9 1 Scope
2 Normative references
3 Definitions
Listeria monocytogenes
enumeration of
4 Principle
4.1 Preparation of the initial suspension in one of the two diluents described, as necessary.
4.2 Resuscitation for�1�h at�20 °˚C.
4.3 Surface plating, on the solid selective culture medium contained in two Petri dishes, of a sp…
4.4 Incubation of the dishes at�!37�°C” and examination after�24�h and�48�h.
4.5 Confirmation of presumptive colonies of
10 4.6 From the number of confirmed colonies, calculation of the number of
5 Culture media and reagents
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven)
6.2 Drying cabinet
6.3 Incubators,
6.4 Water bath,
6.5 Loops
6.6 Glass
6.7 pH-meter,
6.8 Test tubes
6.9 Total-delivery graduated pipettes,
6.10 Petri dishes,
6.11 Jars,
6.12 Gas mixture
6.13 Equipment for the Henry illumination test
6.14 Microscope
7 Sampling
11 8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.3 Enumeration of characteristic colonies
12 9.4 Confirmation of
9.5 Confirmation of
13 9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
14 Inoculation and interpretation of CAMP test plates
Reactions for the identification of Listeria spp.
9.7 Definitive confirmation
15 10 Expression of results
10.1 Counting of colonies of
10.2 Method of calculation
16 11 Precision
12 Quality control of culture media
13 Test report
17 (normative) Diagram of procedure
(normative) Diagram of procedure
(normative) Composition and preparation of media and reagents
(normative) Composition and preparation of media and reagents
Buffered peptone water
Composition
Preparation
18 Base medium for half-Fraser broth with ammonium iron(III) citrate
Base medium for half-Fraser broth
Ammonium iron(III) citrate solution
Preparation of the medium
19 Agar
Base medium
Nalidixic acid solution
Ceftazidime solution
Polymixin B solution
20 Antibiotic supplement
Supplement
Complete medium
21 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
Composition
Preparation
Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
Composition
Preparation
22 Sheep blood agar
Base
Complete base
Carbohydrate utilization broth (rhamnose and xylose)
Base
23 Carbohydrate solutions
Complete media
Motility agar
Composition
Preparation
CAMP (Christie, Atkins, Munch-Paterson) medium and test strains
Base
Sheep blood medium
Complete medium
24 CAMP reaction strains
Hydrogen peroxide solution
Phosphate-buffered saline (PBS)
Composition
Preparation
Sheep blood red cell suspension
(informative) Henry illumination test
(informative) Henry illumination test
25 Examination of plates for suspect colonies
27 (normative) Normative references to international publications with their relevant European publi…
(normative) Normative references to international publications with their relevant European publi…

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