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BS EN ISO 19036:2019

BS EN ISO 19036:2019

$189.07

Microbiology of the food chain. Estimation of measurement uncertainty for quantitative determinations

Published By Publication Date Number of Pages
BSI 2019 48
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This document specifies requirements and gives guidance for the estimation and expression of measurement uncertainty (MU) associated with quantitative results in microbiology of the food chain.

It is applicable to the quantitative analysis of:

  • products intended for human consumption or the feeding of animals;

  • environmental samples in the area of food production and food handling;

  • samples at the stage of primary production.

The quantitative analysis is typically carried out by enumeration of microorganisms using a colony-count technique. This document is also generally applicable to other quantitative analyses, including:

  • most probable number (MPN) techniques;

  • instrumental methods, such as impediometry, adenosine triphosphate (ATP) and flow cytometry;

  • molecular methods, such as methods based on quantitative polymerase chain reaction (qPCR).

The uncertainty estimated by this document does not include systematic effects (bias).

PDF Catalog

PDF Pages PDF Title
2 undefined
4 European foreword
Endorsement notice
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms, definitions and symbols
3.1 Terms and definitions
12 3.2 Symbols
13 4 General considerations
14 5 Technical uncertainty
5.1 Identification of main sources of uncertainty
5.1.1 General aspects
15 5.1.2 Sampling uncertainty
5.1.3 Bias
5.1.4 Critical factors
16 5.2 Estimation of technical uncertainty
5.2.1 General aspects
5.2.2 Reproducibility standard deviation derived from intralaboratory experiments, sIR
21 5.2.3 Reproducibility standard deviation derived from interlaboratory studies
22 6 Matrix uncertainty
6.1 General aspects
23 6.2 Case of homogeneous laboratory (or test) sample
6.3 Multiple test portions from laboratory samples
24 6.4 Known characteristic of the matrix
25 7 Distributional uncertainties
7.1 General aspects
7.2 Colony-count technique — Poisson uncertainty
7.3 Colony-count technique — Confirmation uncertainty
26 7.4 Most probable number uncertainty
27 8 Combined and expanded uncertainty
8.1 Combined standard uncertainty
8.1.1 General considerations
8.1.2 Combined standard uncertainty based on separate technical, matrix, and distributional standard uncertainties
28 8.1.3 Combined standard uncertainty based on reproducibility standard deviation alone
8.2 Expanded uncertainty
8.3 Worked examples
8.3.1 Example 1 — Technical, matrix and Poisson components of uncertainty
8.3.2 Example 2 — Poisson component negligible
29 8.3.3 Example 3 — Poisson, matrix and confirmation components
8.3.4 Example 4 — Technical, matrix and most probable number components
30 9 Expression of measurement uncertainty in the test reports
9.1 General aspects
31 9.2 Results below the limit of quantification
9.2.1 General aspects
9.2.2 Example
33 Annex A (informative) Calculation of standard deviations with two or more than two test portions (intralaboratory reproducibility standard deviation and matrix uncertainty standard deviation)
38 Annex B (informative) Matrix effect and matrix uncertainty
40 Annex C (informative) Intrinsic variability (standard uncertainty) of most probable number estimates
42 Annex D (informative) Correction of experimental standard deviations for unwanted uncertainty components
45 Bibliography

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BS EN ISO 19036:2019
$189.07