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BS ISO 21073:2019

BS ISO 21073:2019

$142.49

Microscopes. Confocal microscopes. Optical data of fluorescence confocal microscopes for biological imaging

Published By Publication Date Number of Pages
BSI 2019 24
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This document specifies commonly used quantities regarding image performance in confocal laser scanning microscopy used for imaging of fluorescent biological specimens.

This document applies only to confocal single point scanners using single photon excitation.

PDF Catalog

PDF Pages PDF Title
2 undefined
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Quantities
4.1 Resolution and strength of optical sectioning
4.1.1 General
11 4.1.2 Definition of resolution
4.1.3 Definition of strength of optical sectioning
4.1.4 Measurement
14 4.2 Uniformity of field and centring accuracy
4.2.1 Definition of uniformity of field and centring accuracy
4.2.2 Measurement
15 4.3 Co-registration accuracy
4.3.1 Definition of co-registration accuracy
16 4.3.2 Measurement of co-registration accuracy
4.4 Stability of illumination power
4.4.1 General
17 4.4.2 Measurement of stability of illumination power
18 4.5 Field number of the confocal scan optic
4.5.1 General
4.5.2 Definition of field number of the confocal scan optic
4.5.3 Measurement of maximum diameter of scanned field
4.6 Scanning frequency
21 Annex A (informative) Theoretical resolution
23 Bibliography

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BS ISO 21073:2019
$142.49