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BSI PD CEN/TS 17717:2022

BSI PD CEN/TS 17717:2022

$102.76

Plant biostimulants. Detection of Salmonella spp.

Published By Publication Date Number of Pages
BSI 2022 22
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This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]: — PFC 6(A): Microbial plant biostimulant; — PFC 6(B): Non-microbial plant biostimulant; — CMC 7: Microorganisms. It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

PDF Catalog

PDF Pages PDF Title
2 undefined
7 1 Scope
2 Normative references
3 Terms and definitions
8 4 Principle
4.1 General
4.2 Enrichment in selective liquid medium
4.3 Plating out on selective solid media
4.4 Confirmation
5 Culture media, reagents, antisera
5.1 General
9 5.2 Isolation chromogenic agar
5.3 Non-selective agar
5.4 Confirmation selective agar
6 Equipment and consumables
10 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
11 9.2 Selective enrichment
9.3 Isolation
9.4 Confirmation
9.4.1 General
9.4.2 Selection of colonies for confirmation
9.4.3 Serological testing
9.4.3.1 General
12 9.4.3.2 Elimination of auto-agglutinable strains
9.4.3.3 Examination for O-antigens
9.4.3.4 Examination for H-antigens
9.4.3.5 Interpretation of serological reactions
13 9.4.4 Selective medium for H2S production
9.4.5 Serotyping
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory studies
14 11.2 Sensitivity
11.3 Specificity
11.4 LOD50
12 Test report
15 Annex A (normative)Diagram of the procedures
16 Annex B (normative)Culture media and reagents
B.1 General
B.2 Buffered peptone water (BPW)
B.2.1 Composition
B.2.2 Preparation
17 B.3 Novobiocin solution
B.3.1 Composition
B.3.2 Preparation
B.4 Nutrient agar (example of non-selective medium)
B.4.1 Composition
B.4.2 Preparation
B.4.3 Preparation of nutrient agar plates
18 B.5 Triple sugar iron agar (TSI agar ; example for H2S production agar)
B.5.1 Composition
B.5.2 Preparation
B.6 Saline solution
B.6.1 Composition
B.6.2 Preparation
19 B.7 Antisera
B.8 Performance testing for the quality assurance of the culture media
20 Annex C (informative)Examples of selective plating-out media

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BSI PD CEN/TS 17717:2022
$102.76