This International Standard specifies a method of detection of Trichinella<\/i> spp. muscle stage larvae in meat of individual animal carcasses intended for human consumption. It is applicable to the examination of meat from domestic and sylvatic animal species, which can be infected by nematodes of the genus Trichinella<\/i>.<\/p>\n
This method does not allow the identification of the species or genotype of detected parasites; species or genotype identification can be carried out by molecular methods.<\/p>\n
The method described in this International Standard is intended to be used in conjunction with the guidelines in the OIE Manual of Diagnostic Tests and Vaccines and by the International Commission on Trichinellosis (ICT) for Trichinella<\/i> testing and the inspection of carcasses intended for human consumption, unless it has been demonstrated by other means that the animal was not at risk for exposure to Trichinella<\/i>.<\/p>\n
The artificial digestion\/magnetic stirrer method is considered to be the standard method because it has proven to give the most reliable results in validation studies.<\/p>\n
Provided equivalence with the method described within this International Standard can be documented, alternative methods can be used for analysis.<\/p>\n<\/div>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 4<\/td>\n | European foreword <\/td>\n<\/tr>\n | ||||||
| 6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 1\tScope 2\tNormative references 3\tTerms and definitions <\/td>\n<\/tr>\n | ||||||
| 10<\/td>\n | 4\tPrinciple 4.1\tGeneral 4.2\tSample size 4.3\tBlending\/grinding of the muscle sample 4.4\tPreparation of the digest fluid 4.5\tDigestion of the chopped meat <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 4.6\tFiltration of the digest fluid 4.7\tSedimentation of the digest fluid 4.8\tMicroscopic examination 4.9\tVerification of findings 5\tReagents <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 6\tApparatus <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 7\tSampling, labelling, and transport 8\tSample preparation 9\tProcedure 9.1\tGeneral 9.2\tBlending\/grinding <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 9.3\tPreparation of the digest fluid 9.4\tDigestion of the chopped meat in the glass beaker 9.5\tFiltration of the digest fluid <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 9.6\tSedimentation of the digest fluid in the separatory funnel 9.7\tCollection of the primary and secondary sediment 9.8\tMicroscopic examination <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 10\tDocumentation 11\tExpression of the results 12\tSafety measures <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | Annex\u00a0A (normative) Sample collection <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | Annex\u00a0B (normative) Frozen samples <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | Annex\u00a0C (informative) Artificial digestion magnetic stirrer method <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | Annex\u00a0D (informative) Example of a laboratory worksheet for recording data when testing pooled samples by digestion assay <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Microbiology of the food chain. Detection of Trichinella larvae in meat by artificial digestion method<\/b><\/p>\n |