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ASTM-E1706:2000 Edition

ASTM-E1706:2000 Edition

$82.33

E1706-00e1 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates

Published By Publication Date Number of Pages
ASTM 2000 118
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ASTM E1706-00e1

Historical Standard: Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates

ASTM E1706

Scope

1.1 This test method covers procedures for testing freshwater organisms in the laboratory to evaluate the toxicity of contaminants associated with whole sediments. Sediments may be collected from the field or spiked with compounds in the laboratory.

1.1.1 Test methods are described for two toxicity test organisms, the amphipod Hyalella azteca ( H. azteca) (see 13.1.2) and the midge Chironomus tentans (C. tentans) (see 14.1.2). The toxicity tests are conducted for 10 days in 300-mL chambers containing 100 mL of sediment and 175 mL of overlying water. Overlying water is renewed daily and test organisms are fed during the toxicity tests. Endpoints for the 10-day toxicity tests are survival and growth. These test methods describe procedures for testing freshwater sediments; however, estuarine sediments (up to 15 ppt salinity) can also be tested with H. azteca. In addition to the 10-day toxicity test method outlined in 13.1.2 and 14.1.2, general procedures are also described for conducting 10-day sediment toxicity tests with H. azteca (see 13.1.2) and C. tentans(see 14.1.2).

1.1.2 Guidance for conducting sediment toxicity tests is outlined in Annex A1 for Chironomus riparius, in Annex A2 for Daphnia magna and Ceriodaphnia dubia, in Annex A3 for Hexagenia spp., in Annex A4 for Tubifex tubifex, and in for the Diporeia spp. Guidance is also provided in Annex A7 for conducting long-term sediment toxicity tests with H. azteca by measuring effects on survival, growth, and reproduction. Guidance is also provided in for conducting long-term sediment toxicity tests with C. tentans by measuring effects on survival, growth, emergence, and reproduction. outlines the data that will be needed before test methods are developed from the guidance outlined in to for these test organisms. General procedures described in Sections 17 for sediment testing with H. azteca and C. tentans are also applicable for sediment testing with the test organisms described in Annex A1 to Annex a7.

1.2 Procedures outlined in this test method are based primarily on procedures described in the United States Environmental Protection Agency (USEPA) (1-8 ) and Guides E 1367, E 1391, E 1525 and E 1688.

1.3 Additional research and methods development are now in progress to: (1) evaluate additional test organisms, (2) further evaluate the use of formulated sediment, (3) refine sediment dilution procedures, (4) refine sediment toxicity identification evaluation (TIE) procedures (9), (5) refine sediment spiking procedures, (6) develop in situ toxicity tests to assess sediment toxicity and bioaccumulation under field conditions, (7) evaluate relative sensitivities of endpoints measured in tests, (8) develop methods for new species, (9) evaluate relationships between toxicity and bioaccumulation, and (10) produce additional data on confirmation of responses in laboratory tests with natural populations of benthic organisms. Some issues that may be considered in interpretation of test results are the subject of continuing research including the influence of feeding on bioavailability, nutritional requirements of the test organisms, and additional performance criteria for organism health. See Section 6 for additional detail. This information will be described in future editions of this standard.

1.4 The USEPA (1) and Guide E 1688 also describes 28-day bioaccumulation methods for the oligochaete Lumbriculus variegatus.

1.5 Results of tests, even those with the same species, using procedures different from those described in the test method may not be comparable and using these different procedures may alter bioavailability. Comparison of results obtained using modified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with aquatic organisms. If tests are conducted with procedures different from those described in this test method, additional tests are required to determine comparability of results. General procedures described in this test method might be useful for conducting tests with other aquatic organisms; however, modifications may be necessary.

Keywords

bioavailability; Ceriodaphnia dubia; Chironomus riparius; Chironomus tentans; contamination; Daphnia magna; Diporeia spp.; Hexagenia spp.; Hyalella azteca; invertebrates; sediment; toxicity; Tubifex tubifex; cladoceran, midge, amphipod, mayfly, oligochaete

ICS Code

ICS Number Code n/a

DOI: 10.1520/E1706-00E01

PDF Catalog

PDF Pages PDF Title
1 Scope
4 Referenced Documents
5 Terminology
TABLE 1
TABLE 2
6 Summary of Standard
8 Significance and Use
10 TABLE 3
TABLE 4
11 Interferences
12 TABLE 5
13 Reagents and Materials
14 TABLE 6
15 TABLE 7
TABLE 8
16 Hazards
17 Facilities, Equipment, and Supplies
18 Sample Collection, Storage, Manipulation, and Characterization
19 TABLE 9
23 Quality Assurance and Quality Control
24 TABLE 10
25 TABLE 11
26 Collection, Culturing, and Maintaining Test Organisms
28 TABLE 12
30 FIG. 1
31 FIG. 2
TABLE 13
32 FIG. 3
FIG. 4
33 Hyalella azteca

TABLE 14

34 TABLE 15
TABLE 16
35 TABLE 17
36 FIG. 5
37 Chironomus tentans

38 FIG. 6
39 FIG. 7
40 TABLE 18
41 TABLE 19
TABLE 20
42 TABLE 21
FIG. 8
43 Calculation
46 TABLE 22
FIG. 9
47 FIG. 10
FIG. 11
FIG. 12
48 FIG. 13
FIG. 14
49 TABLE 23
52 FIG. 15
55 Report
56 Precision and Bias
57 FIG. 16
58 TABLE 24
TABLE 25
59 TABLE 26
TABLE 27
TABLE 28
60 TABLE 29
TABLE 30
61 Keywords
A1. GUIDANCE FOR CONDUCTING SEDIMENT TOXICITY TESTS WITH CHIRONOMUS RIPARIUS
A1.1
62 A1.2
A1.3
TABLE A1.1
63 TABLE A1.2
64 A1.4
A1.5
A1.6
65 A2. GUIDANCE FOR CONDUCTING SEDIMENT TOXICITY TESTS WITH DAPHNIA MAGNA (D. magna) AND CERIODAPHNIA DUBIA ( C. dubia)
A2.1
66 A2.2
A2.3
TABLE A2.1
67 A2.4
A2.5
TABLE A2.2
68 A2.6
69 A3. GUIDANCE FOR CONDUCTING SEDIMENT TOXICITY TESTS WITH HEXAGENIA SPP.
A3.1
A3.2
A3.3
TABLE A3.1
70 TABLE A3.2
71 A3.4
72 A3.5 Toxicity Test Specifications
73 A3.6
A4. GUIDANCE FOR CONDUCTING SEDIMENT TOXICITY TESTS WITH TUBIFEX TUBIFEX
A4.1
TABLE A4.1
74 A4.2
A4.3
75 TABLE A4.2
76 A4.4
A4.5
A4.6
77 A5. GUIDANCE FOR CONDUCTING SEDIMENT TOXICITY TESTS WITH DIPOREIA SPP.
A5.1
A5.2
A5.3 Obtaining Test Organisms
TABLE A5.1
78 TABLE A5.2
79 A5.4
A5.5 Toxicity Test Specifications
80 A5.6
A6. GUIDANCE FOR CONDUCTING A HYALELLA AZTECA 42-DAY TEST FOR MEASURING EFFECTS OF SEDIMENT-ASSOCIATED CONTAMINANTS ON SURVIVAL, GROWTH, AND REPRODUCTION
A6.1
81 A6.2
TABLE A6.1
82 TABLE A6.2
TABLE A6.3
83 A6.3
85 A6.4
86 FIG. A6.1
87 FIG. A6.2
88 FIG. A6.3
TABLE A6.4
89 A7. GUIDANCE FOR A LIFE-CYCLE TEST FOR MEASURING EFFECTS OF SEDIMENT-ASSOCIATED CONTAMINANTS ON CHIRONOMUS TENTANS
A7.1
90 A7.2
TABLE A7.1
91 A7.3
TABLE A7.2
TABLE A7.3
92 FIG. A7.1
93 FIG. A7.2
95 FIG. A7.3
96 FIG. A7.4
97 A7.4
TABLE A7.4
99 FIG. A7.5
FIG. A7.6
100 FIG. A7.7
FIG. A7.8
101 A7.5
102 A8. FOOD PREPARATION
A8.1
A8.2
103 TABLE A8.1
TABLE A8.2
104 A8.3
105 A9. FEEDING RATE FOR THE 10-DAY SEDIMENT TOXICITY TEST METHOD WITH CHIRONOMUS TENTANS
A9.1
A9.2
A9.3
A9.4
A9.5
TABLE A9.1
106 A9.6
REFERENCES
TABLE A9.2
TABLE A9.3

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