šŸ”„šŸ”„ Donā€™t Miss Out on Our End of Autumn Sale. If you have any questions, feel free to click the bottom right corner to contact our customer service..

Concat us[email protected]
Shopping Cart

No products in the cart.

šŸ”
BS EN 15634-1:2019

BS EN 15634-1:2019

$102.76

Foodstuffs. Detection of food allergens by molecular biological methods – General considerations

Published By Publication Date Number of Pages
BSI 2019 20
PDF Format Searchable Printable Preview Now
Guaranteed Safe Checkout
Categories: ,

If you have any questions, feel free to reach out to our online customer service team by clicking on the bottom right corner. Weā€™re here to assist you 24/7.
Email:[email protected]

This document provides the overall framework for detection of sequences corresponding to species containing allergens using the polymerase chain reaction (PCR). It relates to the requirements for the specific amplification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified nucleic acid sequence.

Guidelines, minimum requirements and performance criteria laid down in European Standards are intended to ensure that comparable and reproducible results are obtained in different laboratories. This document has been established for food matrices.

This document is intended to be used in addition to EN 15842.

PDF Catalog

PDF Pages PDF Title
2 National foreword
6 European foreword
7 Introduction
8 1 Scope
2 Normative references
3 Terms and definitions
3.1 Terms relative to extraction and purification of DNA
9 3.2 Terms relative to amplification of DNA
3.3 Definitions referring to controls
10 4 General requirements for laboratories
4.1 General
4.2 Laboratory organization
4.3 Apparatus and equipment
4.4 Material and reagents
11 5 Procedure
5.1 Principle
5.2 General
6 Isolation/Extraction of nucleic acid
6.1 Preparation of sample
6.2 DNA extraction
6.3 DNA quantitation
12 6.4 Quality of DNA
6.5 Storage conditions of extracted DNA
7 Amplification of specific target sequences
7.1 Reagents
7.1.1 Target DNA
7.1.2 Water
7.1.3 Deoxynucleoside triphosphate (dNTPs) solution
7.1.4 PCR buffer solution
13 7.1.5 Thermostable DNA Polymerase
7.1.6 Optional additional reagents
7.2 Primers
7.2.1 Primer design
7.2.2 Forward and reverse primer
7.2.3 Validation of primer sequences
14 7.3 PCR procedure
7.3.1 General
7.3.2 PCR profile and performance criteria
7.3.3 PCR target descriptions
7.3.4 Controls
7.3.5 PCR amplification
15 8 Detection and confirmation of PCR products
8.1 General
8.2 Quantitation of PCR products
8.2.1 General considerations
8.2.2 Specific considerations
9 Interpretation
16 10 Expression of the results
10.1 General
10.2 Expression of a positive result
10.2.1 Qualitative PCR
10.2.2 Quantitative PCR
10.3 Expression of a negative result
17 11 Test report
18 Bibliography

Related products

BS EN 15634-1:2019
$102.76