BS EN 15634-5:2023

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Foodstuffs. Detection of food allergens by molecular biological methods – Mustard (Sinapis alba) and soya (Glycine max). Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR

Published By	Publication Date	Number of Pages
BSI	2023	20

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Categories: 67.120.10 - Meat and meat products, BSI

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Description

This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).

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PDF Pages	PDF Title
2	undefined
7	1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents 5.1 General 5.2 Extraction reagents
8	5.3 DNA purification by means of solid phase extraction
9	5.4 Real-time PCR reagents 6 Apparatus and equipment 6.1 General 6.2 DNA extraction
10	6.3 PCR 7 Procedure 7.1 General 7.2 Sample preparation 7.3 Preparation of extracts 7.3.1 DNA extraction with CTAB and DNA purification
11	7.3.2 Quantification and normalization of DNA concentration
12	7.4 Real-time PCR set-up 7.4.1 Reaction mix for real-time PCR
13	7.4.2 Amplification reagent control 7.4.3 Extraction blank control 7.4.4 Positive extraction control 7.4.5 Temperature/time programme (real-time PCR) 7.4.6 Accept/Reject criteria 7.4.7 Identification
14	8 Validation 8.1 General 8.2 Specificity 8.3 Ring trial validation study 8.3.1 Setup of the ring trial study
15	8.3.2 Ring trial validation results 8.3.2.1 General 8.3.2.2 Results for the positive control dilution series
16	8.3.3 Qualitative interpretation 8.3.3.1 General
18	8.3.3.2 Limit of detection 9 Test report

Additional information

Status	Definitive
Pages	20
Publication Date	2023-05-16
ISBN	978 0 539 18723 6
Standard Number	BS EN 15634-5:2023
Title	Foodstuffs. Detection of food allergens by molecular biological methods – Mustard (Sinapis alba) and soya (Glycine max). Qualitative detection of a specific DNA sequence in cooked sausages by real-time PCR
Identical National Standard Of	EN 15634-5
Replaces	PD CEN/TS 15634-5:2016
Descriptors	Biochemical methods of analysis, Qualitative analysis, Food testing, Allergies, Deoxyribonucleic acid, Food products, Mustard, Biochemistry, Nucleic acids, Sausages
Publisher	BSI
Committee	AW/275
ICS Codes	67.120.10 - Meat and meat products

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BS EN 15634-5:2023

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