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BS EN 17644:2022

BS EN 17644:2022

$102.76

Foodstuffs. Detection of food allergens by liquid chromatography. mass spectrometry (LC-MS) methods. General considerations

Published By Publication Date Number of Pages
BSI 2022 22
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This document establishes an overall framework covering qualitative and quantitative methods for the determination of food allergens and allergenic ingredients using mass spectrometry-based methods for the determination of specific peptides/proteins. This document provides general guidelines and performance criteria applicable to this methodology. Guidelines, minimum requirements and performance criteria laid down in this document are intended to ensure that comparable and reproducible results are obtained by different analysts, instrumentation and laboratories.

PDF Catalog

PDF Pages PDF Title
2 National foreword
5 European foreword
6 Introduction
7 1 Scope
2 Normative references
3 Terms and definitions
10 4 General laboratory requirements
4.1 Principle
4.2 Apparatus and equipment
4.3 Material and reagents
5 Method development
5.1 General
11 5.2 Sample preparation
5.2.1 Grinding
5.2.2 Extraction/purification
5.2.3 Enzymatic digestion
12 5.3 Detection
5.3.1 General
5.3.2 Selection of the target proteins/peptides
13 5.3.3 Selection of measured MRM transitions
14 5.3.4 Internal standard (IS)
6 Method validation procedure
6.1 General
15 6.2 Method validation parameters
6.2.1 Measurand
6.2.2 Limit of detection and limit of quantification
6.3 Selectivity
16 6.4 Calibration curves
6.5 Trueness
6.6 Precision
17 6.7 Measurement uncertainty
6.8 Robustness
6.9 Fit-for-purpose/applicability
7 Routine analysis of allergenic food ingredients
7.1 General
7.2 Validation of an analytical run
18 7.3 Test report
20 Bibliography

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BS EN 17644:2022
$102.76