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BS EN ISO 14501:2021 – TC

$134.27

Tracked Changes. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2021 42
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This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 ?g/kg for milk powder and 0,005 ?g/kg for liquid milk. The limit of quantification (LOQ) is 0,1 ?g/kg for milk powder and 0,01 ?g/kg for liquid milk.

The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.

PDF Catalog

PDF Pages PDF Title
1 30443359
23 A-30404345
24 National foreword
26 European foreword
28 Forewords
31 1 Scope
2 Normative references
3 Terms and definitions
32 4 Principle
5 Reagents
5.1 Immunoaffinity column.
33 5.3 Nitrogen gas.
5.4 Aflatoxin M1 standard solutions.
34 6 Apparatus
35 7 Sampling
8 Procedure
8.1 General
8.2 Preparation of test samples
8.2.1 Milk
8.2.2 Milk powder
8.3 Immunoaffinity column preparation
36 8.4 Test sample purification
8.5 High performance liquid chromatography (HPLC)
8.5.1 Pump setting
8.5.2 Chromatographic performance
8.5.3 Calibration curve of aflatoxin M1
37 8.5.4 Analysis of the purified extracts and injection scheme
9 Calculation and expression of results
9.1 Skimmed milk
9.1.1 Calculation
9.1.2 Expression of results
9.2 Skimmed milk powder
9.2.1 Calculation
38 9.2.2 Expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
39 Annex A (informative) Results of an interlaboratory trial
40 Bibliography
BS EN ISO 14501:2021 - TC
$134.27