BS EN ISO 14501:2021 – TC
$134.27
Tracked Changes. Milk and milk powder. Determination of aflatoxin M1 content. Clean-up by immunoaffinity chromatography and determination by high-performance liquid chromatography
Published By | Publication Date | Number of Pages |
BSI | 2021 | 42 |
This document specifies a method for the determination of aflatoxin M1 content in milk and milk powder. The lowest level of validation is 0,08 µg/kg for whole milk powder, i.e. 0,008 µg/l for reconstituted liquid milk. The limit of detection (LOD) is 0,05 ?g/kg for milk powder and 0,005 ?g/kg for liquid milk. The limit of quantification (LOQ) is 0,1 ?g/kg for milk powder and 0,01 ?g/kg for liquid milk.
The method is also applicable to low-fat milk, skimmed milk, low-fat milk powder and skimmed milk powder.
PDF Catalog
PDF Pages | PDF Title |
---|---|
1 | 30443359 |
23 | A-30404345 |
24 | National foreword |
26 | European foreword |
28 | Forewords |
31 | 1 Scope 2 Normative references 3 Terms and definitions |
32 | 4 Principle 5 Reagents 5.1 Immunoaffinity column. |
33 | 5.3 Nitrogen gas. 5.4 Aflatoxin M1 standard solutions. |
34 | 6 Apparatus |
35 | 7 Sampling 8 Procedure 8.1 General 8.2 Preparation of test samples 8.2.1 Milk 8.2.2 Milk powder 8.3 Immunoaffinity column preparation |
36 | 8.4 Test sample purification 8.5 High performance liquid chromatography (HPLC) 8.5.1 Pump setting 8.5.2 Chromatographic performance 8.5.3 Calibration curve of aflatoxin M1 |
37 | 8.5.4 Analysis of the purified extracts and injection scheme 9 Calculation and expression of results 9.1 Skimmed milk 9.1.1 Calculation 9.1.2 Expression of results 9.2 Skimmed milk powder 9.2.1 Calculation |
38 | 9.2.2 Expression of results 10 Precision 10.1 Interlaboratory test 10.2 Repeatability 10.3 Reproducibility 11 Test report |
39 | Annex A (informative) Results of an interlaboratory trial |
40 | Bibliography |