BS EN ISO 22174:2024
$167.15
Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection and quantification of microorganisms. General requirements and definitions
| Published By | Publication Date | Number of Pages |
| BSI | 2024 | 36 |
This document specifies the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). This document is applicable to the testing for microorganisms and viruses from the food chain using the polymerase chain reaction (PCR). This document, or parts of it, is applicable to other fields of PCR diagnostics based on a case-by-case evaluation. The minimum requirements laid down in this document are intended to ensure that comparable and reproducible results are obtained in different laboratories. This document has been established for microorganisms from the food chain and is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 2 | undefined |
| 10 | 3.1 General terms |
| 11 | 3.2 Terms related to the extraction and purification of DNA/RNA |
| 12 | 3.3 Terms related to reverse transcription of RNA to DNA 3.4 Terms related to DNA amplification by PCR/RT-PCR |
| 13 | 3.5 Terms related to controls |
| 14 | 3.6 Terms related to qPCR |
| 15 | 3.7 Terms related to dPCR |
| 16 | 4.1 General |
| 17 | 4.2 Laboratory sample 4.3 Sampling, transport and storage 4.4 Preparation of test sample 5.1 Microbial enrichment 5.2 Virus concentration |
| 18 | 6.1 General 6.2 Prevention of amplification of DNA from dead cells 6.3 Nucleic acid extraction, release and purification 6.4 Nucleic acid quality and quantity |
| 20 | 9.1 General 9.2 Laboratory setup 9.2.1 General |
| 21 | 9.2.2 Control of flows |
| 22 | 9.2.3 Cleaning of laboratory 9.2.4 Environmental monitoring for nucleic acid contamination |
| 23 | 12.1 Enrichment and sample treatment |
| 24 | 12.2 Amplification 12.2.1 General 12.2.2 Control reaction |
| 26 | 12.2.3 Detection of amplicon 12.2.4 Data analysis |
| 27 | 12.3 Evaluation 12.3.1 Qualitative evaluation |
| 28 | 12.3.2 Quantitative evaluation |
| 29 | 12.4 Test report 14.1 General 14.2 Validation |
| 30 | 14.3 Verification |
